Lyncee Tec Sa

Yves Emery

Etienne Cuche

Christian Depeursinge

Frédéric Montfort

Christophe Moser

Pierre Marquet

Nicolas Aspert

Zahra Monemhaghdoust

Jonas Kuehn

Florian Charriere

Anca Marian

Sébastien Bourquin

Tristan Colomb

Silvain Herminjard

The present disclosure concerns an imaging system for imaging a sample immersed in a controlled environment. The system comprises—at least one enclosure configured to hold at least one imaging sensor or camera inside the enclosure, the enclosure including at least one opening and at least one transparent window for imaging the sample; and—a flexible channel comprising a first extremity and a second extremity, the first extremity being connected to the enclosure at said at least one opening and the second extremity being configured to be connected to a wall of the hermetic chamber, the flexible channel defining or enclosing a passage extending through the flexible channel and to or into the enclosure.

Imaging system for imaging in a controlled environment

An optical fiber splitter device comprising at least two optical fibers of different lengths is disclosed for partial or complete compensation of the optical path difference between waves interfering to generate a hologram or an interferogram. Various implementations of this fiber splitter device are described in apparatuses for holographic and interferometric imaging of microscopic and larger samples.

Fiber splitter device for digital holographic imaging and interferometry and optical system comprising said fiber splitter device

The techniques, apparatus, material and systems are described for a portable camera device which can be attached to the camera port of a conventional transmission or reflection microscope for complex wave front analysis. At least one holographic element (BS, grating) splits the beam (s) containing the sample information in two beams (r,o) and filters (r', o&#x2032;) them. The proposed invention has a relaxed alignment sensitivity to displacement of the beam coming from the microscope. Besides since it compensates the coherence plane tilt angle between reference and object arms, it allows for creating high-visibility interference over the entire field of view. The full-field off-axis holograms provide the whole sample information.

Digital holographic device

A new way of mixing instrumental and digital means is described for the general field of wave front sensing. The present invention describes the use, the definition and the utility of digital operators, called digital wave front operators (DWFO) or digital lenses (DL), specifically designed for the digital processing of wave fronts defined in amplitude and phase. DWFO are of particular interest for correcting undesired wave front deformations induced by instrumental defects or experimental errors. DWFO may be defined using a mathematical model, e.g. a polynomial function, which involves coefficients. The present invention describes automated and semi-automated procedures for calibrating or adjusting the values of these coefficients. These procedures are based on the fitting of mathematical models on reference data extracted from specific regions of a wave front called reference areas, which are characterized by the fact that specimen contributions are a priori known in reference areas. For example, reference areas can be defined in regions where flat surfaces of a specimen produce a constant phase function. The present invention describes also how DWFO can be defined by extracting reference data along one-dimensional (1D) profiles. DWFO can also be defined in order to obtain a flattened representation of non-flat area of a specimen. Several DWFO or DL can be combined, possibly in addition with procedures for calculating numerically the propagation of wave fronts. A DWFO may also be defined experimentally, e.g. by calibration procedures using reference specimens. A method for generating a DWFO by filtering in the Fourier plane is also described. All wave front sensing techniques may benefit from the present invention. The case of a wave front sensor based on digital holography, e.g. a digital holographic microscope (DHM), is described in more details. The use of DWFO improves the performance, in particular speed and precision, and the ease of use of instruments for wave front sensing. The use of DWFO results in instrumental simplifications, costs reductions, and enlarged the field of applications. The present invention defines a new technique for imaging and metrology with a large field of applications in material and life sciences, for research and industrial applications.

Wave front sensing method and apparatus

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Mont-sur-Lausanne, Switzerland

Yves Emery