Fujifilm Manufacturing Europe B.v.

Fuji Photo Film B.v.

Fuji Photo Film Company, Limited

Fuji Photo Manufacturing Europe B.v.

Yuzo Toda

Jan Bastiaan Bouwstra

Andries Johannes Jozef Van Es

Marc Willem Theodoor Werten

Hendrik Wouter Wisselink

Sebastianus Gerardus J M Kluijtmans

Joseph Hubertus Olijve

Makoto Kikuchi

Yoichi Maruyama

Masaaki Miki

Akira Kase

Ronald Wiegers

Frederik Anton De Wolf

Tanja Jacoba Van Den Bosch

Huibert Albertus Van Boxtel

Naohiro Takeda

Gertjan Bögels

Richéle Deodata Wind

Tanja Jacoba Bosch Van Den

Leo Disse

Seimi Satake

Huub Gillissen

Rich&eacute;le Deodata Wind

Toshiyuka Sekiya

Yasuo Iwasa

Frederik Anton Wolf De

Ronaldus J T H Wiegers

Sipke Jan Koopmans

Toshiyuki Sekiya

The invention concerns a cell support comprising an RGD-enriched gelatine that has a more even distribution of RGD sequences than occurring in a natural gelatine and with a minimum level of RGD sequences. More precise the percentage of RGD sequences related to the total number of amino acids is at least 0.4 and if the RGD-enriched gelatine comprises 350 amino acids or more, each stretch of 350 amino acids contains at least one RGD motif. Preferably the RGD-enriched gelatines are prepared by recombinant technology, and have a sequence that is derived from a human gelatine or collagen amino acid sequence. The invention also relates to RGD-enriched gelatines that are used for attachment to integrins. In particular The RGD-enriched gelatines of the invention are suitable for coating a cell culture support for growing anchor-dependant cell types. Further, the RGD-enriched gelatines of the invention may find use in medical applications, in particular as a coating on implant or transplant material or as a component of drug delivery systems.

Use of RGD-enriched gelatine-like proteins for inhibition of cancer metastasis

Method for prevention of platelet aggregation employing and RGD-enriched gelatine-like protein

The invention relates to a support for culturing cells, in particular to microcarriers coated with gelatine or gelatine-like proteins. Such microcarriers serve as support for culturing anchorage dependent cells. In particular the invention relates to a process for the preparation of a cell culture support comprising the step of coating a microcarrier bead with gelatine or gelatine-like protein, said gelatine or gelatine-like protein having a molecular weight of about 40 kDa to about 200 kDa.

Process for coating cell-culture support

RGD-enriched gelatine-like proteins with enhanced cell binding

Drug delivery system comprising an RGD-enriched gelatine

RGD-enriched gelatines

The invention concerns a cell support comprising an RGD-enriched gelatine that has a more even distribution of RGD sequences than occurring in a natural gelatine and with a minimum level of RGD sequences. More precise the percentage of RGD sequences related to the total number of amino acids is at least 0.4 and if the RGD-enriched gelatine comprises 350 amino acids or more, each stretch of 350 amino acids contains at least one RGD motif. Preferably the RGD-enriched gelatines are prepared by recombinant technology, and have a sequence that is derived from a human gelatine or collagen amino acid sequence. The invention also relates to RGD-enriched gelatines that are used for attachment to integrins. In particular The RGD-enriched gelatines of the invention are suitable for coating a cell culture support for growing anchor dependant cell types. Further, the RGD-enriched gelatines of the invention may find use in medical applications, in particular as a coating on implant or transplant material or as a component of drug delivery systems.

The invention relates to compositions suitable for plasma substitution comprising as a plasma expander a recombinant gelatin-like protein. Characteristic is that the gelatin-like protein essentially is free of hydroxyproline. This absence of hydroxyproline prevents the composition from gelling and thus allows the use of high-molecular weight proteins in order to establish a suitable colloid osmotic pressure. Specific advantage of the gelatin-like proteins is that these avoid the risk of anaphylactic shock that exists in conjunction with the use of commercially available preparations.

Recombinant gelatin-like proteins for use as plasma expanders

A color photographic material is disclosed comprising a black image forming coupler substituted in the 2, 4 and/or 6 position shown by formula (I) or formula (II), where in LINK represents a divalent linking group, BALL represents a group that prevents the coupler from diffusing away from the layer containing it. X and Y each represent a hydrogen atom or a coupling-off group capable of being released upon an oxidative coupling reaction with a developing agent. The material has a good color reproduction and excellent light stability.

Photographic colour material containing a resorcinol derivative as black coupler

The invention provides oil-in-water emulsions comprising recombinant collagen-like polymer in an amount sufficient to act as stabiliser of the emulsion. The polymer is especially a polypeptide which is free of helix structure, has an isoelectric point at least 0.5 pH units removed from the pH of the oil-in-water emulsion. Furthermore, amphiphilic recombinant collagen-like polymers are provided for use in oil-in-water emulsions. The amphiphilic polymers are polar at one end as a result of a relative abundance of polar amino acids, and apolar at the other end as a result of a relative abundance of apolar amino acids.

Preparation of oil-in-water emulsions stabilised with recombinant collagen-like material and related products

The invention relates to compositions suitable for plasma substitution comprising as a plasma expander a recombinant gelatin-like protein. Characteristic is that the gelatin-like protein can be a monomer or a polymer like a dimer, trimer or a tetramer of a human recombinant gelatin-like protein having an isolectric point of less than 8. The resulting gelatin-like proteins provide a method to control the clearance rate of a plasma expander by its molecular weight. Preferably the gelatin-like proteins have a low hydroxyproline content which prevents the composition from gelling and thus allows the use of high-molecular weight proteins in order to establish a suitable colloid osmotic pressure. An additional advantage of the gelatin-like proteins is that these avoid the risk of anaphylactic shock that exists in conjunction with the use of commercially available preparations.

Use of recombinant gelatin-like proteins as plasma expanders and compositions suitable for plasma substitution

Silver halide emulsions for photographic uses contain a nucleation peptiser having at least one region A of up to 100 amino acids. Region A can either consist of (i) at least two silver-binding amino acids and up to 98 amino acids between the two silver-binding amino acids, or can consist of (ii) a stretch of from 9 to 100 amino acids having a polarity corresponding to an average transfer free energy &#x394;F equal to or higher than +1.0 kcal/mol. The nucleation peptizer also has at least one region B (separated from region A) having at least 50 amino acids and having a polarity corresponding to an average transfer free energy equal to or lower than &#x2212;1.0 kcal/mol and not containing methionine residues. The emulsion may also contain a growth peptiser having similar characteristics to the nucleation peptiser, but having a longer A region and a greater overall length.

Silver-dispersing polypeptides

The invention is directed to a photocatalytic sheet comprising a support, a photocalalytic layer comprising photocatalyst materials and binder, and at least one functional layer wherein said functional layer is in between said support and said photocatalytic layer, wherein said photocatalytic layer and said functional layer are brought on the support using extrusion coating technology or casting technology, as well as to a photocatalytic film sheet comprising: a photocatalytic layer comprising photocatalyst materials and binder, and at least one functional layer, wherein said film is formed by applying casting technology.

Photocatalytic sheet or film and its manufacturing process

Growing community of inventors

Goirle, Netherlands

Yuzo Toda