E.i. Dupont De Nemours and Company

Alliance for Sustainable Energy, LLC

E.i. Du Pont De Nemours and Company Alliance for Sustainable Energy LLC

Yuying Zhang

Perry G Caimi

Luan Tao

Paul Veikko Viitanen

Min Zhang

Yat-Chen Chou

Mary Ann Franden

Jean-Francois Tomb

Siqun Wang

Mario W Chen

Carol M McCutchen

Laura McCole

Kyle Knoke

Vasantha Nagarajan

Vasantha Nagarajan Nigarajan

Carol Mccutchen

Laura Mccole

Strains ofwere engineered by introducing a chimeric xylose isomerase gene that contains a mutant promoter of theglyceraldehyde-3-phosphate dehydrogenase gene. The promoter directs increased expression of xylose isomerase, and when the strain is in addition engineered for expression of xylulokinase, transaldolase and transketolase, improved utilization of xylose is obtained.

Zymomonas with improved xylose utilization

Identified are mutants of the promoter of theglyceraldehyde-3-phosphate dehydrogenase gene, which direct improved expression levels of operably linked heterologous nucleic acids. These are high expression promoters useful for expression of chimeric genes in, and other related bacteria.

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Through screening of amutant library the himA gene was found to be involved in the inhibitory effect of acetate onperformance. Xylose-utilizingstrains further engineered to reduce activity of the himA gene were found to have increased ethanol production in comparison to a parental strain, when cultured in mixed-sugars medium comprising xylose, and, in particular, in the presence of acetate.

Through screening of amutant library the himA gene was found to be involved in the inhibitory effect of acetate onperformance. Xylose-utilizingfurther engineered to reduce activity of the himA gene were found to have increased ethanol production in comparison to a parental strain, when cultured in medium comprising xylose and acetate.

This invention relates to a method for utilizing less purified starch in fermentation processes. One example is a recombinantcontaining a exogenous extracellular isoamylase activity that is capable of utilizing small oligomers containing (1,6) linkages (including but not limited to isomaltose and panose) in fermentations to produce useful products. The invention is useful in large-scale industrial biofermentations by reducing the cost of the substrate carbohydrate.

&#x3b1;(1,6)-linked glucose oligosaccharide hydrolyzing enzyme polynucleotides and polypeptides and methods of use thereof

Alpha(1,6)-linked glucose oligosaccharide hydrolyzing enzyme polynucleotides and polypeptides and methods of use thereof

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New Hope, PA, United States of America

Yuying Zhang