Guangdong Provincial Bioengineering Institute (Guangzhou Sugarcane Industry Research Institute)

Institute of Nanfan & Seed Industry, Guangdong Academy of Sciences

Yuxing An

Jian Zeng

Qingfu Wang

Huiyi He

Fangye Lao

Yuanxian Xu

Weiwei Zhang

Lina Fan

Jinrong Li

Lei Liang

Zhongxing Huang

Qiuping Ling

Donglei Sun

The present invention relates to a method for conducting high-throughput and directed mutagenesis for sugarcane resistance to glyphosate by plasma. The method is as follows: sugarcane embryonic calli are irradiated by a plasma instrument under a sterile condition for mutagenesis, wherein the mutagenesis power is 140&#x2dc;200 W, the discharging distance is 35&#x2dc;45 mm, the mutagenesis time is 110&#x2dc;140 s and the protective gas is nitrogen; buffering culture, moderate/high concentration of glyphosate stress screening, differentiation into seedlings, glyphosate stress screening of bottle seedlings and stress screening via spraying glyphosate on the leave surfaces of potted plants are conducted for the treated calli. The present invention has the advantages of safe operation, simplicity, practicability, high handling capacity, low contamination, and due to implementation of directed stress screening, high screening efficiency, decreased subsequent screening workload and visual identification of resistant mutant strains.

Method for conducting high-throughput and directed mutagenesis for sugarcane resistance to glyphosate by plasma

A food packaging cup comprises a cup body, an inner film bag disposed in the cup body and a cup lid structure for sealing a cup mouth of the cup body. A bag mouth of the inner film bag is connected with the cup body by a detachable structure, a liquid receiving inner cavity is formed inside the inner film bag, the inner film bag can be separated from the inner wall of the cup body, and an air hole communicated with a gap between the inner film bag and the cup body is formed on the cup body. The food packaging cup is simple in structural production, low in cost, and convenient for various classes of consumers to further drink up thick fruit juice, milk, fermented milk, yoghourt and the like, thereby avoiding residues in the cup. Moreover, garbage of two different materials can be sorted clearly.

Food packing cup

Growing community of inventors

Guangzhou, China

Yuxing An