Asymchem Life Science (tianjin) Co., Ltd

Asymchem Life Science Co., Ltd.

Yuxia Cui

Na Zhang

Jiadong Zhao

Jiangping Lu

Hao Hong

Gage James

Yulei Ma

Wenyan Yu

Xin Huang

Yibing Cheng

Yi Xiao

Xingfu Xu

Yanyan Gao

Vyasarayani Williams Rajasekar

Mingmin Hao

Xuewu Dong

Disclosed are a modified epoxy resin immobilized enzyme, a preparation method therefor and an application thereof. Herein, the preparation method includes the following steps: modifying an epoxy resin, adding a polyethyleneimine to a modified epoxy resin for further modification, and then adding an enzyme to be immobilized and a glutaraldehyde for immobilization, to obtain the modified epoxy resin immobilized enzyme. The epoxy resin is modified, the polyethyleneimine is added to the modified epoxy resin for the further modification, and an aldehyde group in the resin and an amino group in the polyethyleneimine are covalently bound to each enzyme, then it is activated by the bifunctional reagent glutaraldehyde.

Modified epoxy resin immobilized enzyme, preparation method therefor and application thereof

The invention provides a transaminase mutant and application thereof, wherein the amino acid sequence of the transaminase mutant is formed after mutation of the amino acid sequence as shown in SEQ ID NO: 1, and mutated amino acid sites comprise T7C+S47C sites. The transaminase mutant having the mutation sites can be further prepared into an immobilized enzyme through an immobilization technology, the immobilized enzyme has relatively high activity and high stability, can be recycled for multiple times, and is applicable to continuous flow reaction in a packed bed.

Transaminase mutant and application thereof

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Tianjin, China

Yuxia Cui