Other

Wuhan Yangyang Yuanlv Mini-Reservoirs Co., Ltd.

Yuwei Wang

Rui Hou

Chen Chen

Xiaoping Chen

Binhao Zhang

Zhimin Liu

Cailing Lu

Junsheng Cao

Bixiang Zhang

Huifang Liang

Mengyi Cao

Zhanguo Zhang

A broom and a manufacturing method thereof, the broom comprising at least a broom handle () and a broom core; the bottom of the broom handle () is provided with a transverse plate (); the broom core is fixed on the transverse plate (), and comprises more than one bundle of brushes; the brushes are provided with dispersed slender resilient strips () thereon for cleaning the ground; the resilient strips () take up more than &#xbd; the length of the brushes; the side surface of the resilient strips () is arc-shaped, and tapers from top to bottom; the bottom end of the resilient strips () is a thickened layer. The brushes of the broom are made by cutting waste beverage bottles, solving the problem of environmental pollution caused by the waste beverage bottles; the manufacturing method is simple, thus reducing the production cost; the bottom end of the resilient strips () is a thickened layer (), thus significantly improving wear resistance and stability and greatly extending service life compared to a bamboo broom.

Broom and manufacturing method thereof

A method for establishing an animal model of hepatocellular carcinoma (HCC) bone metastasis, the method including: 1) establishing 97H and LM3 cell clones with stable expression of firefly luciferase (LUC); 2) allowing HCC cells to form bone metastasis in nude mice via intratibial injection; 3) reproducing HCC bone metastasis in nude mice via intracardiac injection of tumor cells; and 4) isolating a sub-population of tumor cells that targets metastasis to bone. The 97H and the LM3 are highly metastatic HCC cell lines transfected with luciferase gene. BALB/cA-nu mice are 4-5 weeks old and maintained in laminar flow cabinets under SPF conditions and received human care throughout an entire study. A cell number for intratibial injection is 0.5&#xd7;10, and a cell number for intracardiac injection is 1&#xd7;10.

Methods for establishing animal model of hepatocellular carcinoma bone metastasis

Growing community of inventors

Wuhan, China

Yuwei Wang