Ramot at Tel-aviv University Ltd.

Tsinghua University

Technion Research & Development Foundation Limited

Yissum Research Development Company of the Hebrew University of Jerusalem Ltd.

Rheinisch-Westfälische Technische Hochschule (Rwth) Aaachen

Yuval Ebenstein

Micha Fridman

Elmar Weinhold

Assaf Grunwald

Uri Banin

Amit Meller

Tal Gilboa

Wenjun Jiang

Ting Zhu

Tslil Gabrieli

Taleb Mokari

Chen Torfstein

Yael Michaeli-Hoch

Chunbo Lou

Xuejin Zhao

Shahar Zirkin

Sapir Margalit

Yael Michaeli Hoch

A method of detecting an epigenetic DNA modification is described herein, the method comprising: attaching to DNA a labeling agent selective for the DNA modification; contacting an aqueous solution comprising the DNA with at least one addressable region on a surface of a substrate, wherein the addressable region comprises a positively charged substance capable of attaching the DNA to the surface, and the addressable region is surrounded by a hydrophobic region; and determining an amount of labeling agent in the addressable region. Further described herein is a kit comprising a substrate comprising discrete hydrophilic regions separated by a hydrophobic region, and a labeling agent selective for an epigenetic DNA modification; as well as an article of manufacture comprising a substrate comprising a discrete regions separated by a hydrophobic region, a positively charged substance and DNA attached thereto, the DNA comprising such a labeling agent.

Multi-sample array chip for DNA modification quantification

Provided herein is a method of labeling the epigenetic modification-hydroxymethyl-cytosine along a DNA molecule that involves attaching a-hydroxymethyl-cytosine specific labeling agent to the DNA molecule, which is effected by enzymatically synthesizing UDP--N-Glucose by subjecting-azidoglucose to an enzymatic catalysis by N-acetylhexoseamine-kinase (NahK) in the presence of ATP to thereby obtain a phosphorylated-azidoglucose, followed by subjecting the phosphorylated-azidoglucose to enzymatic catalysis by uridyltransferase (GlmU) in the presence of UTP to thereby obtain UDP--N-Glucose, followed by incubating the DNA molecule with UDP--N-Glucose and ß-glucosyltransferase, to thereby obtain a-hydroxymethyl-cytosin e glycosylated by a-azidoglucose residue in the DNA molecule, and reacting the modified DNA molecule with a labeling agent which is chemically compatible with the azide group of the-azidoglucose residue.

Detection of hydroxymethylcytosine bases

A method of DNA fragmentation is provided. The method comprising incubating a semi-solid biological sample which comprises the DNA with an auxiliary domain-directed nuclease having a binding affinity and selectivity to pre-defined sites in the DNA so as to yield a DNA fragment-of-interest, to thereby fragment the DNA.

Methods and kits for fragmenting DNA

Methods and kits for detecting the presence of at least one target DNA sequence with or without a modification in a DNA molecule are provided.

Methods of detecting modified and unmodified DNA

Methodologies for labeling the epigenetic modification 5-hydroxymethylcytosine (5hmC) along a DNA molecule, and for determining a presence or a level of this epigenetic modification based on a ratio of fluorescence intensity of a labeled DNA sample to absorption intensity of the DNA sample at 260 nm are disclosed. Related compositions and reagents, and methods of preparing same are also disclosed.

A method of diagnosing a disease associated with a DNA repeat sequence is disclosed. The method comprises:

Analysis of methylation status and copy number

Methodologies for labeling the epigenetic modification 5-hydroxymethyl-cytosine (5hmC) along a DNA molecule, and for imaging this epigenetic modification along a DNA molecule are disclosed. Related compositions and reagents, and methods of preparing same are also disclosed.

The present invention provides probes functionalized with nanoparticles, methods for binding nanoparticles to the probes and the use of such probes in nanometer scale imaging techniques. More specifically, the present invention provides a tip device having at least a portion thereof with an outer surface bound to at least one sub-layer of a material comprising nanoparticles, the nanoparticles acting as donors, acceptors, modifiers, quenchers and/or enhancers with respect to electromagnetic radiation.

Growing community of inventors

Yavne, Israel

Yuval Ebenstein