Other

Kowa Company Limited

National Institute for Agro-environmental Sciences Independent Administrative Institute

National Institute for Agro-environmental Sciences Independent Administration Institute

National Institute for Agro-environmental Science Independent Administrative Institute

Yuuichi Yoshioka

Kazuhiro Takagi

Naoki Harada

The present invention provides a stable complex microbial system, which simultaneously decomposes a plurality of organic contaminants even under a polluted environment with these contaminants and permits more effective decomposition of persistent organic contaminants such as PCNB and simazine. A support for holding a complexed accumulation of degrading bacteria, which contains a porous material provided as a support on which a degrading bacterium A capable of degrading at least one organic contaminant and a degrading bacterium B capable of degrading another organic contaminant are accumulated, is produced. The degrading bacterium A may be a PCNB-degrading bacterium, particularly a degrading bacterium containing a degrading bacterium having part or all of mycological characteristics ofsp. PD653 and the degrading bacterium B may be a degrading bacterium containing a degrading bacterium having part or all of mycological characteristics of &#x3b2;-CDB21.

Method of decontaminating polluted environments with bacteria on a porous support

The present invention provides a stable complex microbial system, which simultaneously decomposes a plurality of organic contaminants even under a polluted environment with these contaminants and permits more effective decomposition of persistent organic contaminants such as PCNB and simazine. A support for holding a complexed enrichment of degrading bacteria, which contains a porous material provided as a support on which degrading bacteria A capable of degrading at least one organic contaminant and degrading bacteria B capable of degrading another organic contaminant are enriched, is produced. The degrading bacteria A may be a PCNB-degrading bacteria, particularly degrading bacteria containing degrading bacteria having part or all of the bacteriological characteristics ofsp. PD653 and the degrading bacteria B may be degrading bacteria containing degrading bacteria having part or all of the bacteriological characteristics of &#x3b2;-Proteobacteria CDB21.

[object Object]

Restoring soil and preventing contamination of ground water

It is an object of the invention to improve a conventional soil percolation technique to thereby provide a method of enriching and isolating decomposing bacteria decomposing an organochlorine agricultural chemical PCNB which is difficult to decompose, in a short time period, and to provide decomposing bacteria for efficiently processing PCNB. To practice the method, an enrichment soil layer is formed by mixing a soil containing an organochlorine agricultural chemical PCNB with a fragmented porous material having an infinite number of micropores and at the same time a greater adsorptivity for adsorbing PCNB than the soil, and an inorganic salt medium containing a carbon and nitrogen source, the carbon and nitrogen source being formed by only PCNB, is circulated through the enrichment soil layer , thereby enriching the aerobic bacteria in the fragmented porous material.

Method for enriching and isolating bacteria which aerobically degrades PCNB

A porous material having a greater adsorptivity for adsorbing organic contaminants, such as agricultural chemicals, than a target soil, is fragmented. The fragmented porous material is mixed into a soil which the decomposing bacteria inhabit, thereby forming an enrichment soil layer. Through the enrichment soil layer, there is circulated an inorganic salt medium containing carbon and nitrogen sources formed by only an organic contaminant to be decomposed, thereby rapidly enriching the decomposing bacteria in the fragmented porous material. The fragmented porous material is inoculated into new fragmented porous material to form an enrichment layer consisting of the fragmented porous material only. Into the enrichment layer, the inorganic salt medium containing carbon and nitrogen sources formed by only an organic contaminant to be decomposed is circulated and the operation of enriching decomposing bacteria is repeated a plurality of times for the new fragmented porous material as well to enhance the degree of purity and enrichment of the decomposing bacteria, thereby rapidly isolating the decomposing bacteria.

Growing community of inventors

Kochi, Japan

Yuuichi Yoshioka