Jsr Life Sciences Corporation

Jsr Corporation

Jsr Micro N.v.

Rion Company Ltd.

Yuuichi Ueya

Tsutomu Nakajima

Kazuma Sekimoto

Takashi Sannomiya

Masaru Ueno

Miyuki Fukuda

Hirokazu Kai

Yasuhito Satou

Kousuke Hiroki

Yuki Oohashi

This invention relates to a method of producing a probe-bound carrier, a probe-bound carrier and a method of detecting or separating a target substance. The method of producing a probe-bound carrier includes: step 1 of mixing a carrier having tosyl groups and a probe; and step 2 of reducing the amount of tosyl groups on a surface of a carrier, in which a proportion of area S2 that is occupied by one tosyl group on a surface of the carrier obtained in the step 2 with respect to area S1A that is occupied by one tosyl group on a surface of the carrier used in the step 1 (S2/S1A&#xd7;100%) is not less than 140%.

Method of producing probe-bound carrier, probe-bound carrier and method of detecting or separating target substance

The present invention relates to a magnetic particle dispersion, and the magnetic particle dispersion includes a magnetic particle, an isothiazoline compound, and an aqueous medium.

Magnetic particle dispersion

Provided is a solid phase carrier which has high water dispersibility, allows facilitated binding of a ligand to a reactive functional group, and exhibits suppressed non-specific adsorption, and with which, in the case of using the solid phase carrier by having a ligand bound thereto, for example, detection of a target substance can be carried out with high sensitivity and low noise. Disclosed is a solid phase carrier having bound thereto a polymer including a structural unit represented by the following Formula (1) and a structural unit represented by the following Formula (2): wherein in Formula (1), Rrepresents a hydrogen atom or a methyl group; and Rrepresents an organic group having a zwitterionic structure,  in Formula (2), Rrepresents a hydrogen atom or a methyl group Rrepresents &#x2014;(C&#x2550;O)&#x2014;O&#x2014;*, &#x2014;(C&#x2550;O)&#x2014;NR&#x2014;* (wherein Rrepresents a hydrogen atom or a methyl group; and the symbol * represents a position of bonding to Rin Formula (2)), or a phenylene group; in a case in which Rrepresents &#x2014;(C&#x2550;O)&#x2014;O&#x2014;*, Rrepresents a hydrogen atom, or an organic group having a reactive functional group, and in a case in which Rrepresents &#x2014;(C&#x2550;O)&#x2014;NR&#x2014;* or a phenylene group, Rrepresents an organic group having a reactive functional group, provided that Ris not an organic group having a zwitterionic structure.

Solid phase carrier, ligand-bound solid phase carrier, method for detecting or separating target substance, and method for producing solid phase carrier

A solid-phase support, formed by binding a chain polymer at least to the surface, wherein the chain polymer is a block polymer comprising a first block constituted from repetitions of a hydrophilic structural unit and a second block constituted from repetitions of a structural unit having a reactive functional group, a content ratio of the number of moles 'a' of the reactive functional group contained in the chain polymer and the number of moles 'b' of the whole structural unit contained in the chain polymer, (a/b), is from 0.03 to 0.25, and a density of the chain polymer occupying the surface of the solid-phase support is 0.1 polymers/nmor more.

Solid-phase support, ligand-binding solid-phase support, method for detecting or separating target substance, and method for producing the solid-phase support

To provide a solid-phase carrier to which impurities are hard to nonspecifically adsorb. A solid-phase carrier, formed by binding a chain polymer, wherein the chain polymer comprises a random polymer structure containing a first structural unit having a reactive functional group, and a second structural unit having no reactive functional group or having a reactive functional group having a reactivity lower than that of the reactive functional group of the first structural unit, and the content ratio of the number of moles 'a' of the reactive functional group contained in the first structural unit to the number of moles 'b' of the entire structural unit contained in the chain polymer, (a/b), is from 0.01 to 0.7.

Solid support, ligand-bound solid support, detection or separation method for target substance, solid support production method, and ligand-bound solid support production method

Growing community of inventors

Minato-ku, Japan

Yuuichi Ueya