Kao Corporation

Yutaro Baba

Junichi Sumitani

Shuji Tani

Takashi Kawaguchi

Nozomu Shibata

Hiroshi Kakeshita

Provided is a mutant &#x3b2;-glucosidase capable of more efficiently saccharifying biomass. A mutant &#x3b2;-glucosidase comprising an amino acid sequence having at least 80% sequence identity to SEQ ID NO: 1, wherein the amino acid sequence has asparagine at one or more positions selected from the group consisting of positions corresponding to positions 787, 790, and 797 of SEQ ID NO: 1, and has &#x3b2;-glucosidase activity.

Mutant &#x3b2;-glucosidase

Provision of a mutant &#x3b2;-glucosidase having high protease resistance. A mutant &#x3b2;-glucosidase consists of an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 1, wherein the amino acid sequence comprises Gln and Thr respectively at positions corresponding to positions 649 and 655 of SEQ ID NO: 1 or Tyr-Glu-Pro-Ala-Ser-Gly in a region corresponding to the region from position 653 to position 658 of SEQ ID NO: 1, and has &#x3b2;-glucosidase activity.

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Toyama, Japan

Yutaro Baba