Nippon Oil Company

Yutaka Oguchi

Ichiro Kojima

Tetsuo Satoh

Kenji Maruhashi

Noboru Endoh

Takashi Suzuki

Tetsuya Takezono

Masao Imaizumi

Hiroo Takeda

Tatsuo Kinoshita

Takaaki Amari

Takeshi Kanda

Kouji Komiya

Naomasa Asano

A process for producing vitamin B.sub.12 by the fermentation technique which comprises cultivating a vitamin B.sub.12 -producing microorganism belonging to the genus Propionibacterium in a culture medium containing a carbon source and a nitrogen source, and collecting vitamin B.sub.12 accumulated in the cells of the microorganism; characterized in that

Process for producing vitamin B.sub.12 by the fermentation technique,

A process for separating porphyrins, which comprises

Process for separating porphyrins

A process for separating vitamin B.sub.12 in pure form, which comprises bringing a vitamin B.sub.12 -containing liquid containing impurities into contact with a resin selected from the group consisting of (A) a divinylbenzene/styrene copolymer having a mode pore diameter of at least about 200 A and a total pore volume of more than 0.6 ml/g, and (B) a copolymer of divinylbenzene/styrene/an unsaturated alkyl ester of an aromatic polycarboxylic acid represented by the formula ##STR1## wherein R represents a C.sub.3 -C.sub.10 unsaturated alkyl moiety having a carbon-carbon double bond and n is 2 or 3, said copolymer (B) having a surface area of at least about 700 m.sup.2 /g, to cause vitamin B.sub.12 to be adsorbed to said resin, and eluting the vitamin B.sub.12 with an eluent, and collecting the active eluate.

Process for purifying and separating vitamin B.sub.12

A process for isolating and recovering butene-1 of high purity at a high yield is provided. The process comprises the steps of continuously passing a butane-butene fraction containing 0.1 to 15 wt % of isobutylene and 10 to 50 wt % of butene-1 through a first reactor packed with a strongly acidic cation exchange resin having an average particle size of from 0.2 to 10 mm at a temperature of from 30.degree. to 100.degree. C. and at a space velocity of liquid of from 0.1 to 50 hr.sup.-1 under a pressure of from 1 to 50 atm., dividing the output mixture flowing out of said first reactor into two flows at a division ratio in flow rate of 1.about.15:1, recirculating the first flow having the flow rate of 1.about.15 into said first reactor packed with said cation exchange resin, passing the second flow having the flow rate of 1 through a second reactor packed with a strongly acidic cation exchange resin under the conditions similar to those of the first reactor, distilling the output flow from said second reactor to separate the same into a heavy hydrocarbon fraction containing oligomers of isobutylene and a light hydrocarbon fraction containing butane and butene, and rectifying said light hydrocarbon fraction to isolate butene-1 from other C.sub.4 -hydrocarbons.

Process for isolating and recovering butene-1 of high purity

A fluid consisting of either a liquid or a mixture of a liquid and a gas is intimately contacted with solid particles by introducing the fluid into a contacting vessel to form a fluidized layer of the solid particles. A bed of porous packing is also placed in the contacting vessel and the upper end of the fluidized layer of solid particles is located within the porous packed bed.

Process for fluidized contact

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Tokyo, Japan

Yutaka Oguchi