Sysmex Corporation

Yutaka Maeda

Yasuhiro Kouchi

Hironori Kobayashi

Junyi Ding

Shuji Yamashita

Michitaka Notoya

Mika Yoshimura

Soichi Oue

Takayuki Koshihara

A sample scraping method and a sample scraping device capable of efficiently scraping and collecting a biological tissue section without waste while suppressing contamination is provided. In a sample scraping method for scraping and collecting a biological tissue section held on a slide, the FFPE section held on the slide is heated, and the heated FFPE section is scraped by a blade such that the FFPE section that has been scraped off and remains on the blade is collected in a container.

Sample scraping method and sample scraping device

Disclosed is a nucleic acid amplification method. The method comprises: performing a first nucleic acid amplification using a target nucleic acid contained in a sample as a template; and performing a second nucleic acid amplification using the amplification product produced in the first nucleic acid amplification step as a template. In the first nucleic acid amplification, a DNA polymerase that selectively amplifies a nucleic acid not comprising a uracil base is used to produce an amplification product that does not comprise a uracil base. In the second nucleic acid amplification, (i) dUTP and/or a primer comprising a uracil base, and (ii) a DNA polymerase capable of amplifying a nucleic acid comprising a uracil base are used to produce an amplification product that comprises a uracil base.

Nucleic acid amplification method producing uracil-containing amplification products

Growing community of inventors

Kobe, Japan

Yutaka Maeda