Ajinomoto Co., Ltd.

Nof Corporation

Hokkaido University

Chiba University

Yuta Nakai

Takuji Ueda

Hidetaka Akita

Kota Tange

Hideyoshi Harashima

Tomoko Suzuki

Yoshihiro Usuda

Kazuhiko Matsui

Mika Moriya

Shintaro Iwatani

Yoshiya Gunji

Yuji Joe

Rie Takikawa

Stephen Van Dien

Hiroki Tanaka

Yuichiro Tsuji

Naoya Miura

Hiroyuki Kojima

Hisao Ito

Hiroki Yoshioka

Osamu Kurahashi

Yoshio Kawahara

Kazuhiro Kubo

Kazuo Nakanishi

Hiroto Hatakeyama

Masaki Ota

Shota Sasaki

Shinya Tamagawa

Yuki Noguchi

Tatsunari Takahashi

Manami Konishi

Nae Takata

Yuri Miyata

Ayaka Watanabe

Ryohei Togashi

Mio Maeta

Nayuta Shimizu

The invention provides a cationic lipid, a lipid membrane structure containing same, and use thereof. The cationic lipid is represented by the formula (1) wherein R, R, R, R, R, R, X, X, Y, Y, Z, and Zare as defined in the specification.

Cationic lipid exhibiting improved intracellular dynamics

The present invention provides an O/W type emulsion having a volume median diameter of not more than 100 nm and containing a compound represented by the formula (1) wherein Xand Xare each independently X, Xor a 1,4-piperazinediyl group; s is 1 or 2,Ris an alkyl group having 1-6 carbon atoms,nand nare each independently 0 or 1,R, R, Rand Rare each independently an alkylene group having 1-6 carbon atoms,Yand Yare each independently an ester bond, or the like, andRand Rare each independently a liposoluble vitamin residue or the like.

O/W type emulsion

The present invention aims to provide a cationic lipid that can be used as a nucleic acid delivery carrier, a lipid membrane structure using a cationic lipid, a nucleic acid-introducing agent using a cationic lipid, and a method of achieving nucleic acid introduction by using a nucleic acid-introducing agent containing a cationic lipid. A lipid membrane structure containing a cationic lipid represented by the formula (1) wherein each symbol is as defined in the DESCRIPTION, is superior in the stability in blood and tumor accumulation property. A nucleic acid-introducing agent using the cationic lipid can achieve high nucleic acid delivery efficiency of nucleic acid to be delivered into the cytoplasm.

Cationic lipid

The invention provides a cationic lipid capable of achieving higher intracellular delivery efficiency than conventional cationic lipids, when used as a lipid membrane structure which is a carrier for delivering functional nucleic acid. The cationic lipid is represented by the formula (1): 

Cationic lipid for nucleic acid delivery

In a method for producing a target substance utilizing a microorganism comprising culturing the microorganism in a medium to produce and cause accumulation of the target substance in the medium and collecting the target substance, there is used, as the microorganism, a mutant strain or a genetic recombinant strain constructed from a parent strain of the microorganism having a respiratory chain pathway of high energy efficiency and a respiratory chain pathway of low energy efficiency as respiratory chain pathways, and having either one or both of the following characteristics:

Method for producing substance utilizing microorganism

L-amino acids are produced by culturing a microorganism which has an ability to produce the L-amino acid, but has been modified so that expression of the ybjE gene has been enhanced. The L-amino acid is collected from the culture medium or from the microorganism.

L-amino acid-producing microorganism and method for producing L-amino acid

A bacterium belonging to the genuswhich has an ability to produce L-lysine or L-threonine and which is modified so that a malic enzyme does not function normally in a cell, and a method for producing L-lysine or L-threonine, comprising culturing the bacterium in a medium to produce and cause accumulation of L-lysine or L-threonine, and collecting the L-lysine or L-threonine from the medium.

Method for producing L-lysine or L-threonine

Growing community of inventors

Kawasaki, Japan

Yuta Nakai