Tokyo Electron Limited

Sinfonia Technology Co., Ltd.

Tocalo Co., Ltd.

Asahi Rubber Inc.

Mitsui Chemicals, Inc.

Yusuke Yoda

Akihiro Yamamoto

Kenichi Kagawa

Shigenori Ozaki

Toyohisa Tsuruda

Masato Hosaka

Hirotsugu Shiraiwa

Hisashi Gomi

Tatsuya Hamaguchi

Tomoaki Kurakazu

Shinichi Gomi

Kunitada Hatabayashi

Shinya Miki

Tsutomu Takano

Kakeru Chigama

Yuya Ubukata

Yasuhiro Shibata

The present disclosure provides a method for determining the undifferentiated state of pluripotent stem cells, comprising: irradiating a test culture medium in which pluripotent stem cells are cultured with wavelength light having a wavelength in the range of 190 nm to 2,500 nm or a partial range thereof; detecting the reflected light, transmitted light or transmitted reflected light thereof to obtain absorbance spectrum data; and analyzing the absorbance at all or part of the measurement wavelengths in the absorbance spectrum data to determine the undifferentiated state of the pluripotent stem cells.

Method for determining undifferentiated state of pluripotent stem cell, method for subculturing pluripotent stem cell, and device for use in the methods

An apparatus includes: a processed-image acquisition part for acquiring a processed image including image information of a surface of a substrate having a target film formed thereon; an estimation part for estimating a shape characteristic value of the target film, by applying an estimation model for estimating the shape characteristic value of the target film to the processed image; an underlying influence model creation part for creating an underlying influence model associated with a correlation between a difference between an estimation result of the shape characteristic value of the target film and a shape characteristic value of the target film acquired without using the estimation model, and information related to a color of a surface of an underlying substrate before the target film is formed, and an estimated-result correction part for correcting the estimation result of the shape characteristic value of the target film based on the underlying influence model.

Shape characteristic value estimation apparatus, shape characteristic value estimation method, and storage medium

The purpose of the present invention is to provide a culture container capable of preventing a concave meniscus without using a jig. In order to achieve this purpose, the present invention provides a culture container (A) provided with: a base () having a concave portion (); and a water-repellent layer () formed on an outer edge region () of the bottom () of the concave portion () and the inner circumferential surface () of the concave portion (). One of the surfaces of the water-repellent layer () is exposed to the space in the concave portion ().

Culture container and cell culturing method and cell observation method using culture container

A cell-holding container comprises: an elastic body for holding cells including at least any one of adherent cells at least one selected from the group consisting of stem cells, progenitor cells, somatic cells and germ cells, and suspended cells at least one selected from the group consisting of blood cells, T cells and B cells; and the elastic body is formed of a rubber material containing a rubber component including an additional crosslinking silicone rubber and is able to hold the cells.

Cell-holding container and cell culture method using same

Culture tray

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Tokyo, Japan

Yusuke Yoda