Biopharm Gesellschaft Zur Biotechnologischen Entwicklung Pharmaka Mbh

Aventis Pharma S.a.

Biopharm Gesellschaft Zur Biotechnologieschen Entwicklung Von Pharmaka Mbh

Yusuke Satoh

Michio Kimura

Mieko Katsuura

Shinji Kawai

Fusao Makishima

Hiroyuki Takamatsu

Hideo Miki

Koichi Enomoto

Tomoaki Matsumoto

Kunikazu Sakai

A protein having amino acid sequence in SEQ ID No.:1 of the Sequence Listing derived from human MP52, and a dimer protein thereof. A homodimer protein described above can be obtained by constructing a plasmid containing DNA coding amino acid sequence in SEQ ID No.:1 of the Sequence Listing with a methionine at the N-terminus, introducing the plasmid intofor transformation, solubilizing inclusion bodies obtained by culturing the transformant, purifying the monomer protein from the solubilized solution, refolding the monomer protein into a dimer protein and purifying the same. The homodimer protein described above is useful as a pharmaceutical composition for treating cartilage and bone diseases.

Protein and process for preparing the same

A protein having amino acid sequence in SEQ ID NO: 1 of the Sequence Listing derived from human MP52, and a dimer protein thereof. A homodimer protein described above can be obtained by constructing a plasmid containing DNA coding amino acid sequence in SEQ ID NO: 1 of the Sequence Listing with a methionine at the N-terminus, introducing the plasmid intofor transformation, solubilizing inclusion bodies obtained by culturing the transformant, purifying the monomer protein from the solubilized solution, refolding the monomer protein into a dimer protein and purifying the same. The homodimer protein described above is useful as a pharmaceutical composition for treating cartilage and bone diseases.

A protein consisting of the amino acid sequence in SEQ ID No: 1 derived from human MP52 and a homodimer protein thereof useful for treating cartilage and/or bone diseases and a process for its preparation.

Protein and process for producing the same

Barbituric acid derivative and preventive and therapeutic agent for bone and cartilage containing the same

Growing community of inventors

Saitama, Japan

Yusuke Satoh