Hokkaido University

Nitto-denko Corporation

Yusuke Sato

Hideyoshi Harashima

Manabu Tokeshi

Masatoshi Maeki

Takuya Shishido

Yoshiki Maeda

Sachiko Sakamoto

Hiroto Hatakeyama

Masamitsu Taguchi

Takashi Nakamura

Mamoru Hyodo

Shota Warashina

Kazuki Hashiba

Ayaka Otsu

The present invention addresses the problem of providing lipid nanoparticles which function as gene transfer carriers capable of selective transfer to the liver or spleen. Lipid nanoparticles which contain a pH-sensitive cationic lipid represented by formula (I) [a represents an integer of 3-5; b represents 0 or 1; Rand Reach independently represent a group represented by general formula (A) (Rand Reach independently represent a linear or branched Calkyl group; c represents 0 or 1; v represents an integer of 4-12); and X represents a group represented by general formula (B)(d represents an integer of 0-3; and Rand Reach independently represent a Calkyl group or Calkenyl group, while Rand Rmay form a 5- to 7-membered non-aromatic heterocycle) or represents a 5- to 7-membered non-aromatic heterocyclic group]. (I) (R)(R)C(OH)—(CH)a-(O—CO)b-X. (A): (R)(R)—CH—(CO—O)c-(CH)v-. (B):—(CH)d-N(R)(R).(R)(R)C(OH)—(CH)—(O—CO)-X  (I)(R)(R)—CH—(CO—O)—(CH)-  (A)—(CH)-N(R)(R)  (B)

Lipid nanoparticle

The present invention addresses the problem of providing a lipid nanoparticle in which a nucleic acid, etc., required in genome editing is encapsulated and which can be produced by an alcohol dilution method using flow channels and contributes to high genome editing efficiency. The present invention pertains to a lipid nanoparticle which comprises a lipid component, a DNA nuclease, a guide RNA and a single-stranded oligonucleotide, wherein: the lipid component comprises a pH-sensitive cationic lipid, a neutral phospholipid and a polyalkylene glycol-modified lipid; the ratio of the pH-sensitive cationic lipid relative to the total lipids constituting the lipid nanoparticle is 30-50 mol %; the ratio of the neutral phospholipid relative to the total lipids constituting the lipid nanoparticle is 20-50 mol %; and the ratio of the polyalkylene glycol-modified lipid relative to the total lipids constituting the lipid nanoparticle is 1-4 mol %.

Provided are: a flow channel structure with which lipid particles or micelles, which are useful as nano-sized carriers, for example, in drug delivery systems, are produced with good control of particle size; and a method for forming lipid particles or micelles using the same. Said flow channel structure has a two-dimensional structure such as one in which multiple structural elements (baffles) of a specified width are alternately disposed from the two side faces in a micro-sized flow channel through which feedstock solutions are flowed.

Flow channel structure and lipid particle or micelle formation method using same

A lipid membrane structure includes, as lipid components, a lipid compound represented by Formula (I):(R)(R)C(OH)&#x2014;(CH)&#x2014;(O&#x2014;CO)&#x2014;X&#x2003;&#x2003;(I)

Lipid membrane structure for delivery into siRNA cell

A lipid membrane structure encapsulating an siRNA inside thereof and containing a lipid compound of the formula (I) as a lipid component (Rand Rrepresent CH&#x2014;(CH)&#x2014;CH&#x2550;CH&#x2014;CH&#x2014;CH&#x2550;CH&#x2014;(CH)&#x2014;, n represents an integer of 3 to 5, m represents an integer of 6 to 10, p represents an integer of 2 to 7, and Rand Rrepresent a Calkyl group or a Calkenyl group.

Lipid membrane structure for intracellular delivery of siRNA

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Hokkaido, Japan

Yusuke Sato