Japan Science and Technology Agency

Yusho Kato

Hirohide Saito

Tan Inoue

Kenichi Yoshikawa

Ayako Yamada

Toru Yamanaka

An object of the present invention is to construct an mRNA which specifically responds to a short RNA sequence and can activate, repress, and regulate the translation of the desired gene, and to construct an artificial cell model system using a liposome comprising the mRNA and a cell-free translational system encapsulated therein. The present invention provides: an mRNA comprising a target RNA-binding site located immediately 5' to the ribosome-binding site, and a nucleotide sequence located 5&#x2032; to the target RNA-binding site, the nucleotide sequence being complementary to the ribosome-binding site; an mRNA comprising a small RNA-binding site located 3&#x2032; to the start codon, and a nucleotide sequence located 3&#x2032; to the small RNA-binding site, the nucleotide sequence encoding a protein; and a liposome comprising any of these mRNAs encapsulated therein.

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Hyogo, Japan

Yusho Kato