Sysmex Corporation

Yuriko Egashira

Yukiko Higa

Atsushi Fukunaga

Yukiko Kataoka

Masatoshi Suganuma

Tomofumi Nakada

Tsuyoshi Nomura

Koji Fukagawa

Disclosed is a method for improving affinity of an antibody for an antigen, the method comprising: in the antibody, changing at least 3 amino acid residues of framework region 3 (FR3) as defined by Kabat method to arginine residues or lysine residues, thereby improving affinity of an antibody for an antigen as compared with that of an antibody before said at least 3 amino acid residues are changed to arginine residues or lysine residues, wherein said at least 3 amino acid residues comprise at least 3 selected from the group consisting of amino acid residues at positions 68, 70, 72, 74, 75, 77, 79, 81, 82A, 82B, 83, 84, 85 and 87 of a heavy chain as defined by the Kabat method.

Method for improving affinity of antibody for antigen and use thereof

Disclosed is a monoclonal antibody reactive with a glycopeptide shown in (a), and not reactive with a glycopeptide shown in (b) 

Method for detecting AFP

Disclosed is a monoclonal antibody reacting with a glycopeptide, wherein the glycopeptide contains a core fucose moiety and at least 4 contiguous amino acid residues that are located on the C-terminal side of a glycosylated asparagine moiety, and both of the core fucose moiety in the glycopeptide and an amino acid residue that is located apart by at least three amino acid residues from the C-terminal of the glycosylated asparagine moiety in the glycopeptide are epitopes for the antibody.

Monoclonal antibody reacting with glycopeptide, and use thereof

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Tokorozawa, Japan

Yuriko Egashira