Kagami Inc.

Shiseido Company, Limited

Kanazawa University

Yurika Miyoshi

Masashi Mita

Kenji Hamase

Yusuke Nakade

Kengo Furuichi

Yasunori Iwata

Takashi Wada

Akinori Hara

Maiko Nakane

Shoto Ishigo

Norihiro Sakai

Tadashi Ueda

The present invention addresses the problem of developing an analytical method which makes it possible to diagnose early or mild renal disorders. The method is based on calculating a disease-state index value for renal disorders on the basis of the quantities of D-form and/or L-form amino acids, from feces or intestinal content. By comparing the disease-state index value with a threshold value determined from the disease-state index values of a renal failure patient group and a healthy subject group, it is possible to diagnose a mild renal disorder patient group.

Disease-state biomarker for renal disease

A chiral analysis method of a bound amino acid includes a step of hydrolyzing a bound amino acid using a deuterium chloride-deuterium oxide solution and/or deuterium oxide; a step of separating, by chiral separation, a D-form and an L-form of an amino acid generated by the hydrolyzation; a step of generating fragments from the separated amino acid; and a step of selecting and analyzing a predetermined fragment that contains an &#x3b1; carbon and does not contain a side chain from the generated fragments, by mass spectrometry.

Chiral analysis method of bound amino acid and chiral analysis system

The present invention addresses the problem of identifying a biomarker of renal failure, said biomarker being available from urine or blood, and fluctuating from an early stage than glomerular filtration rate and serum creatinine level, and thus developing a technique for diagnosing early stage kidney failure. A method for analyzing the blood, plasma, serum or urine of a renal failure suspected subject comprises a step of measuring the concentration of a pair of D-form and L-form of at least one amino acid selected from the amino acid group consisting of [D-serine] and [L-serine], etc., contained in the blood, plasma, serum or urine of the subject, and calculating, as an pathological index of the subject, the ratio of the D-form concentration to the L-form concentration or the percentage of the D-form concentration relative to the total concentration of the D-form and L-form.

Marker for early diagnosis of kidney failure

Growing community of inventors

Fukuoka, Japan

Yurika Miyoshi