Icon Genetics Gmbh

Nomad Bioscience Gmbh

Bayer Cropscience Nv

University of Southampton

Icon Genetics Ag

Yuri Gleba

Victor Klimyuk

Sylvestre Marillonnet

Stefan Werner

Serik Eliby

Anatoly Giritch

Carola Engler

Peter Ivanov

Yurii Dorokhov

Anatoli Giritch

Maxim Vladimirovich Skulachev

Romy Kandzia

Anett Stephan

Franziska Jarczowski

Simone Hahn

Joseph Atabekov

Steve Schulz

Frank Thieme

Newell Bascomb

Tobias Schneider

Patrick Roemer

Stefan Herz

Natalia Savelyeva

Stefan Mühlbauer

Mario Gils

Sylvestre Marillonet

Gregor Benning

Mark Bossie

Gerald Hall, Jr

Thomas J Petty, Ii

Heike Prochaska

Meinhart Zenk

Warayut Chotprakaikiat

Valentin Negrouk

Luisa Bortesi

Doreen Tiede

Anja Nickstadt

Duc Bui-Minh

Freda K Stevenson

Carola Thoeringer

Viktor Klimyuk

Anton Shvarts

Yuri Symonenko

Doreen Bartels

Anka THÜMMLER

Anka Thummler

Stefan Muehlbauer

The present invention relates to bacteriocins for control of(salmocins). The bacteriocins are derived from. The salmocins can be expressed in plants and can be used in a method of preventing or reducing infection or contamination of an object with

Bacteriocins for control of

A process of transiently transfecting a plant or leaves on a plant, comprising contacting said plant or said leaves with a suspension comprisingcells of strain CryX or a derivative strain of strain CryX, wherein said derivative strain has the chromosomal background of strain CryX or said derivative strain contains the vir plasmid of strain CryX or a derivative of said vir plasmid.

Agrobacterium for transient transfection of whole plants

A process of producing a recombinant glycoprotein in a plant, in cells of a plant, or in plant cells is provided. The process comprises expressing in said plant, in cells of said plant or in said plant cells a nucleic acid sequence encoding a polypeptide having an N-glycosylation site and co-expressing a nucleic acid sequence encoding a heterologous single-subunit oligosaccharyltransferase.

Method of co-expressing a glycoprotein and a single-subunit olicosaccharyltransferase in a plant

A process of expressing a sequence of interest in a plant, plant part, or plant cell culture, comprising: (a) providing a plant, plant part, or plant cell culture containing in cell nuclei a heterologous DNA having a sequence encoding an RNA replicon operably linked or linkable to a transcription promoter, wherein said sequence encoding an RNA replicon contains (i) sequences for replicon function of said RNA replicon, said sequences being derived from a sequence of a plant RNA virus, (ii) a sequence of interest, whereby said sequences for replicon function exhibit at selected localities of said sequences of said plant RNA virus function-conservative differences from said sequence of said plant RNA virus, said differences causing an increased frequency of replicon formation compared to an RNA replicon not exhibiting said differences; and (b) causing expression of said sequence of interest.

RNA virus-derived plant expression system

The invention provides a method of preventing or reducing contamination of an object such as food with enterohaemorrhagic(EHEC), comprising contacting said object with colicin M or a derivative thereof.

Colicins for the control of EHEC

Protein conjugate comprising a protein antigen for generating an immune response against the HER2/neu protein and an immunogenic carrier covalently bonded to said protein antigen, wherein said protein antigen

HER2/Neu cancer vaccine

A process of endowing a plant or plant cells with a trait of interest by expressing an RNA sequence of interest, said process comprising: providing plant cells or cells of said plant with a first vector and a second vector and selecting cells endowed with said trait of interest, wherein said first vector contains a first nucleotide sequence with a first segment coding, in 5' to 3&#x2032; direction, for&#x2014;a 5&#x2032; part of said RNA sequence of interest and&#x2014;a 5&#x2032; part of an intron; and said second vector contains a second nucleotide sequence with a second segment coding, in 5&#x2032; to 3&#x2032; direction, for&#x2014;a 3&#x2032; part of an intron and&#x2014;a 3&#x2032; part of said RNA sequence of interest.

Plant transformation with in vivo assembly of a trait

A process of expressing a sequence of interest in a plant, plant part, or plant cell culture, comprising: (a) providing a plant, plant part, or plant cell culture containing in cell nuclei a heterologous DNA having a sequence encoding an RNA replicon operably linked or linkable to a transcription promoter, wherein said sequence encoding an RNA replicon contains (i) sequences for replicon function of said RNA replicon, said sequences being derived from a sequence of a plant RNA virus, (ii) a sequence of interest, whereby said sequences for replicon function exhibit at selected localities of said sequences of said plant RNA virus function conservative differences from said sequence of said plant RNA virus, said differences causing an increased frequency of replicon formation compared to an RNA replicon not exhibiting said differences; and (b) causing expression of said sequence of interest.

A process of producing transgenic plants or plant cells stably transformed on a chromosome with a DNA sequence of interest capable of expressing a function of interest, said process comprising (a) providing plant cells or plants with at least two different vectors that are adapted to recombine with each other between site-specific recombination sites compatible with a site-specific recombinase that is also provided in order to produce a non-replicating recombination product containing said DNA sequence of interest, (ii) said at least two different vectors are adapted for integrating said DNA sequence of interest into said chromosome, (iii) said DNA sequence of interest contains sequence portions from at least two of said at least two different vectors, said sequence portions being necessary for expressing said function of interest from said DNA sequence of interest; and (b) selecting plants or plant cells expressing said function of interest.

Plant transformation with in vivo assembly of a sequence of interest using a site-specific recombinase

Process of producing in a plant, in plant tissue, or in plant cells a hetero-oligomeric protein comprising at least a first and a second protein subunit, said process comprising expressing in plant cells at least said first and said second protein subunit by (i) providing to said plant, said plant tissue or said plant cells a plus-sense single-stranded RNA viral vector encoding at least said first and said second protein subunit or (ii) providing to said plant, said plant tissue or said plant cells a first and a second plus-sense single-stranded RNA viral vector, said first viral vector encoding at least said first protein subunit, said second viral vector encoding at least said second protein subunit, whereby at least said first viral vector and said second viral vector are non-competing viral vectors.

Production of hetero-oligomeric proteins in plants

A process of producing one or more than one protein of interest, comprising: (a) providing a plant or plant cells comprising a first heterologous nucleotide sequence comprising a nucleotide sequence encoding an RNA replicon, and a first inducible promoter operably linked to said nucleotide sequence encoding said RNA replicon; said RNA replicon not encoding a protein providing for cell-to-cell movement of said RNA replicon in said plant; said RNA replicon encoding a polymerase and said one or more than one protein of interest, said polymerase being adapted for replicating said RNA replicon; and (b) inducing, in said plant or plant cells of step (a), said inducible promoter, thereby producing said one or more than one protein of interest in said plant or plant cells.

Plant virus-based inducible expression system

A process of producing transgenic multi-cellular plants or parts thereof expressing a trait of interest that has a controlled distribution of said trait to progeny, comprising (i) producing a first plant or a cell thereof having in a first locus of a nuclear chromosome a first heterologous nucleotide sequence comprising a first fragment of a nucleotide sequence encoding said trait of interest, (ii) producing a second plant or a cell thereof having in a second locus of a nuclear chromosome homologous to said nuclear chromosome of step (i), a second heterologous nucleotide sequence comprising a second fragment of the nucleotide sequence encoding said trait of interest, and (iii) hybridising said first and said second plants or cells thereof to generate progeny exhibiting said functional trait of interest. Also disclosed is a process of producing hybrid seeds for agriculture.

Transgenic plants with controlled distribution of a trait to progeny

A process for replicating or for replicating and expressing a sequence of interest in a plant, comprising: (i) an RNA replicon or a precursor thereof, said RNA replicon being derived from a plus-sense single stranded RNA virus and comprising at least one sequence of interest; and (ii) a helper replicon, or a precursor thereof, wherein said helper replicon is (a) incapable of systemic movement in said plant both in the presence and in the absence of said RNA replicon (i) and (b) capable of expressing in a plant one or more proteins necessary for systemic movement of said RNA replicon (i), whereby said RNA replicon (i) is capable of replicating or replicating and expressing said sequence of interest in said plant, but unable to move systemically in said plant in the absence of said one or more proteins expressed by said helper replicon (ii).

Two-component RNA virus-derived plant expression system

Nucleic acid comprising or encoding an RNA replica comprising, in this order, the following segments (i) to (iii): i) a nucleic acid sequence encoding a potexvirus RNA-dependent RNA polymerase or a function-conservative variant thereof; ii) a nucleic acid sequence comprising: a) a potexvirus triple gene block or a function-conservative variant thereof and b) a sequence encoding a potexviral coat protein or a function-conservative variant thereof; or a sequence encoding a tobago viral movement protein; and iii) a heterologous nucleic acid sequence expressible from said replica in a plant or in plant tissue.

Potexvirus-derived replicon

Growing community of inventors

Halle/Saale, Germany

Yuri Gleba