Monsanto Technology LLC

Monsanto Company

Sequenom, Inc.

Genetrace Systems, Inc.

Laboratory Corporation of America Holdings

Ecogen, Inc.

Althea Technologies, Inc.

Yuping Tan

William P Donovan

Annette C Slaney

Mark J Rupar

James Arthur Baum

Thomas M Malvar

Joseph Albert Monforte

Thomas Andrew Shaler

Chih-Rei Chu

Elizabeth Pease

Christopher Hank Becker

Christine S Jany

Jose M Gonzalez

Ahmed Chenna

Jose M Gonzalez, Jr

Syed Hasan Tahir

Jos E Gonz Alez, Jr

Linda Wong

Kathy Stephens

Christine Loehrlein

Dan Pollart

Youssouf Badal

The invention relates to an enhanced method of detecting and/or quantifying at least one analyte in a sample. 

Enhanced method for detecting and/or quantifying an analyte in a sample

Disclosed are novel insecticidal polypeptides, and compositions comprising these polypeptides, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that contain nucleic acid segments that encode the disclosed &#x3b4;-endotoxin polypeptides. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising these polynucleotide sequences, as well as methods for controlling an insect population, such as Colorado potato beetle, southern corn rootworm and western corn rootworm, and for conferring to a plant resistance to a target insect species.

Coleopteran-toxic polypeptide compositions and insect-resistant transgenic plants

Disclosed is a novel &#x3b4;-endotoxin, designated CryET29, that exhibits insecticidal activity against siphonapteran insects, including larvae of the cat flea (), as well as against coleopteran insects, including the southern corn rootworm (), western corn rootworm (), Colorado potato beetle (), Japanese beetle (), and red flour beetle (). Also disclosed are nucleic acid segments encoding CryET29, recombinant vectors, host cells, and transgenic plants comprising a cryET29 DNA segment. Methods for making and using the disclosed protein and nucleic acid segments are disclosed as well as assays and diagnostic kits for detecting cryET29 and CryET29 sequences in vivo and in vitro.

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Polynucleotide encoding coleopteran-toxic polypeptide

Coleopteran-toxic polypeptide compositions

Disclosed are transgenic plants, seeds, and progeny from such plants comprising a cryET29 DNA segment. The cryET29 DNA segment encodes CryET29 crystal protein which comprises SEQ ID NO:2 and exhibits insecticidal activity against siphonapteran insects, including larvae of the cat flea (), as well as against coleopteran insects, including the southern corn rootworm (), western corn rootworm (), Colorado potato beetle (), Japanese beetle (), and red flour beetle ().

Plants transformed with CryET29-encoding nucleic acids

Disclosed are nucleic acids encoding novel insecticidal &#x3b4;-endotoxin polypeptides, and vectors, transformed host cells, and transgenic plants that contain the nucleic acids. Also disclosed are methods of making and using transgenic cells comprising these polynucleotide sequences, as well as methods for controlling an insect population, such as Colorado potato beetle, southern corn rootworm and western corn rootworm, and for conferring to a plant resistance to a target insect species.

Nucleic acids encoding coleopteran-toxic polypeptides and insect-resistant transgenic plants comprising them

Disclosed is a novel &dgr;-endotoxin, designated CryET29, that exhibits insecticidal activity against siphonapteran insects, including larvae of the cat flea ( ), as well as against coleopteran insects, including the southern corn rootworm ( ), western corn rootworm ( ), Colorado potato beetle ( ), Japanese beetle ( ), and red flour beetle ( ). Also disclosed are nucleic acid segments encoding CryET29, recombinant vectors, host cells, and transgenic plants comprising a cryET29 DNA segment. Methods for making and using the disclosed protein and nucleic acid segments are disclosed as well as assays and diagnostic kits for detecting cryET29 and CryET29 sequences in vivo and in vitro.

Bacillus thuringiensis CryET29 compositions toxic to coleopteran insects and ctenocephalides SPP

This invention provides methods, compositions and kits for gene expression analysis and gene expression profiling. The methods of the invention are highly sensitive; have a wide dynamic range; are rapid and inexpensive; have a high throughput; and allow the simultaneous differential analysis of a defined set of genes. The methods, compositions and kits of the invention also provide tools for gene expression data collection and relational data analysis.

Methods for analysis of gene expression

This invention relates to methods for screening nucleic acids for polymorphisms by analyzing amplified target nucleic acids using mass spectrometric techniques and to procedures for improving mass resolution and mass accuracy of these methods of detecting polymorphisms.

Methods of preparing nucleic acids for mass spectrometric analysis

Disclosed are novel insecticidal polypeptides, and compositions comprising these polypeptides, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that contain nucleic acid segments that encode the disclosed &dgr;-endotoxin polypeptides. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising these polynucleotide sequences, as well as methods for controlling an insect population, such as Colorado potato beetle, southern corn rootworm and western corn rootworm, and for conferring to a plant resistance to a target insect species.

Bacillus thuringiensis CryET29 compositions toxic to coleopteran insects and Ctenocephalides SPP

This invention relates to methods for screening nucleic acids for mutations by analyzing nonrandomly fragmented nucleic acids using mass spectrometric techniques and to procedures for improving mass resolution and mass accuracy of these methods of detecting mutations.

Methods of screening nucleic acids using volatile salts in mass spectrometry

Disclosed is a novel .delta.-endotoxin, designated CryET29, that exhibits insecticidal activity against siphonapteran insects, including larvae of the cat flea (Ctenocephalides felis), as well as against colcopteran insects, including the southern corn rootworm (Diabrotica undecimpunctata), western corn rootworm (D. virgifera), Colorado potato beetle (Leptinotarsa decemlineata), Japanese beetle (Popillia japonica), and red flour beetle (Tribolium castaneur). Also disclosed are nucleic acid segments encoding CryET29, recombinant vectors, host cells, and transgenic plants comprising a cryET29 DNA segment. Methods for making and using the disclosed protein and nucleic acid segments are disclosed as well as assays and diagnostic kits for detecting cryET29 and CryET29 sequences in vivo and in vitro.

Bacillus thuringiensis CryET29 compositions toxic to coleopteran insects

This invention relates to methods for screening nucleic acids for mutations by analyzing nonrandomly fragmented nucleic acids using mass spectrometric techniques and to procedures for improving mass resolution and mass accuracy of these methods of detecting

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Fremont, CA, United States of America

Yuping Tan