National Science Council of Republic of China

Yunn B Yang

Ying Chun Tsai

Ching P Tseng

Tsai, Ying C

Hsiang L Hu

Chyuan S Lin

A process for making D-aminoacylse includes adding 1% N-acetyl-DL-amino acid preferably N-acetyl-DL-methionine and N-acetyl-DL-leucine in a culturing medium incubated with bacteria selected from the strain of Alcaligenes faecalis for culturing the bacteria and for inductively promoting an enzyme reaction to produce the D-aminoacylase which is able to hydrolyze D-amino acids and unable to hydrolyze L-amino acids.

Process for making D-aminoacylase

A process for making L-aminoacylase includes a cultivation of microorganism selected from a specy of Alcaligenes, especially the Alcaligenes denitrificans DA 181, and a separation of a produced L-aminoacylase from the bacterial cells for obtaining the L-aminoacylase which may be further purified for the production of L-amino acid. The acylase made by such a process may have an increased stability, beneficial for its commercial and medical values.

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Taipei, Taiwan

Yunn B Yang