Korea Institute of Science and Technology

Yunje Kim

Youngsoon Um

Han Min Woo

Gyeong Taek Gong

Sun Mi Lee

Kyung Min Lee

Gyeongtaek Gong

Hyun Jeong Lee

Eun Mi Kim

Sun-young Choi

Jeong Myeong Ha

Ki Yeon Kim

Jun Won Chwa

Jung Seok Lee

Dongjin Suh

The present specification discloses a transformedstrain which may directly produce squalene from carbon dioxide, and a method for producing squalene and a method for removing carbon dioxide, using the same. In an aspect, the strain may produce squalene using carbon dioxide as a carbon source. Thestrain is economically efficient because a high-value added squalene is produced using light and carbon dioxide present in the atmosphere as a carbon source, and the method for producing squalene is eco-friendly because the strain may be utilized to remove or reduce carbon dioxide in the atmosphere by using microorganisms. The strain of the present disclosure may produce only squalene, which is a desired target material with high purity, and has an advantage in that squalene may be continuously mass-produced.

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The present disclosure relates to a hydrolysate of a mixture of lignocellulosic biomass and seaweed biomass. By mixing seaweed biomass with lignocellulosic biomass and then preparing a hydrolysate, lignocellulosic biomass-derived acetic acid is consumed together with seaweed biomass-derived mannitol. As a result, high sugar productivity can be maintained while reducing fermentation inhibitors. Because the present disclosure can solve the problem of lignocellulosic biomass of decreased fermentation efficiency due to lignocellulose-derived fermentation inhibitors and the problem of seaweed biomass of very low productivity in spite of long fermentation time, the hydrolysate according to the present disclosure may be used to produce biofuels and biochemicals economically.

Hydrolysate of mixture of seaweed biomass and lignocellulosic biomass to improve biochemical and biofuel production, and preparation using the same

The present disclosure discloses a transformedstrain capable of producing biodiesel directly from carbon dioxide and a method for producing biodiesel and a method for removing carbon dioxide using the same. In an aspect, the transformedstrain of the present disclosure can produce biodiesel in large scale using carbon dioxide as a carbon source. Thestrain is environment-friendly because it can be used to remove or reduce carbon dioxide in the atmosphere. The strain of the present disclosure is advantageous in that it can produce biodiesel in large scale because it grows faster and exhibits excellent carbon dioxide fixation capability as compared to other photosynthetic microorganisms.

The present disclosure discloses a vector that can be used for both cyanobacteria and, which contains, sequentially, a pUC replication origin as a replication origin; a spectinomycin-resistant gene as a selection marker; and a promoter selected from a group consisting of a trc promoter, a tetA promoter or a modified tetA promoter, a BAD promoter and a cbbL promoter. An industrially useful substance may be produced effectively using a host cell transformed with the vector. Also, the vector may be used to insert a variety of target genes through simple combination and, as a result, various vectors can be prepared effectively.

Expression vector for cyanobacteria

Disclosed herein are an expression vector capable of expressing myrcene, anstrain transformed with the vector and having improved capability of producing myrcene and a method for producing myrcene and a method for recycling glycerol using the same. In an aspect, the transformedstrain of the present disclosure can produce myrcene with high purity on a large scale using glycerol or glucose as a carbon source. Also, thestrain of the present disclosure is economical and environment-friendly because it can produce high value-added myrcene using waste glycerol as a carbon source. In addition, the strongly volatile myrcene can be produced and isolated at the same time.

Vectors and strains for producing myrcene and method of producing myrcene using the same

Provided is a method for preparing a lignocellulosic biomass hydrolysate having toxicity reduced or removed and being used for fermentation by microorganisms, comprising the steps of: pretreating, hydrolyzing, and using an electrochemical detoxification method.

Electrochemical detoxification method of wood-based hydrolysate for producing biochemicals or biofuels

Disclosed is a shuttle vector that can be used forand, containing: a repressor selected from a group consisting of a lacI repressor and a tetR repressor; a promoter selected from a group consisting of a trc promoter, a tetA promoter and a LacUV5 promoter; a replication origin pBL1 derived from; and a replication origin ColE1 of. A host cell transformed with the shuttle vector can effectively produce industrially useful substances. Also, the shuttle vector may be used to easily insert various combinations of target genes and, as a result, a variety of vectors can be prepared effectively.

Growing community of inventors

Seoul, South Korea

Yunje Kim