Korea Research Institute of Bioscience and Biotechnology

Gwangju Institute of Science and Technology

Yun Ju Sung

Min-Gon Kim

Yong Beom Shin

Hyou-Arm Joung

Min Soo Kim

Ju Young Byun

Hyo Arm Joung

Young-Kyoung Oh

Jun Hyoung Ahn

Disclosed herein are a method for manufacturing a multi-functional bio-material conjugate used as a biosensor for detecting microorganisms, and the like, and a multi-functional bio-material conjugate manufactured by means of the same. The method for manufacturing a multi-functional bio-material conjugate includes: (a) coating a first nanoparticle having magnetic or fluorescent characteristics with protein; (b) manufacturing a conjugate by adsorbing a second nanoparticle having metallic characteristics onto the first nanoparticle coated with protein; and (c) manufacturing the multi-functional bio-material conjugate by adsorbing a bio-material onto the conjugate. The method for manufacturing a multi-functional bio-material conjugate according to the present invention may prevent precipitation of the nanoparticles, easily immobilize the bio-material, and manufacture a bio-material conjugate having multiple functions, by using two kinds of the particles. In addition, the multi-functional bio-material conjugate manufactured by the present method may be used to detect microorganisms at up to a concentration of 10cfu.

Method for manufacturing multi-functional bio-material conjugate using two kinds of particle, and multi-functional bio-material conjugate manufactured by means of same

A membrane electrode includes a novel sensor combining a filtering function of a membrane and a signal measuring ability of an electrode. A target material may be measured by filtration through the membrane. A small amount of target materials may be detected with high sensitivity using an amplified electrical signal by increasing electrical conductivity by reducing metal ions on the membrane, and thus the target material may be subject to quantitative analysis. In addition, only a target material selectively binding to a receptor may be filtrated by passing a sample through the membrane after a receptor material is fixed to the electrode, and thus may be used to detect an electrical signal. In addition, the sensor may measure a signal in various methods such as electrical conductivity, impedance, etc.

Membrane phase electrode using printing and bio-molecule detection using same

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Daejeon, South Korea

Yun Ju Sung