Food Industry Research and Development Institute

Yun-Huey Lee

Wen-Shen Chu

Li-Lin Chen

Chiou-Yen Wen

Ming-Chu Chen

Wen-Hwei Hsu

Yi-Jang Lee

Gwo-Jen Liao

This invention relates to a D-amino acid oxidase of the genus Rhodosporidium and a gene encoding it.

Rhodosporidium D-amino acid oxidase

A method of screening for a given enzyme in colony-forming cells using a substrate of the enzyme that has low solubility. Use of such a method leads to the discovery of glutaryl-7-aminocephalosporanic acid (GL-7-ACA) acylase of Pseudomonas nitroreducens. Also disclosed is a method of obtaining GL-7-ACA acylase from Pseudomonas cells.

Screening and characterization of glutaryl-7-aminocephalosporanic acid

A method of converting cephalosporin C to glutaryl-7-aminocephalosporanic acid. The method including obtaining a cell preparation from a microorganism which produces D-amino acid oxidase, the preparation being a cell-free extract or a suspension of permeated cells; adding a D-.alpha.-amino acid to the preparation; after the adding step, heating the preparation at 50.degree.-75.degree. C. for 5-60 minutes; and incubating cephalosporin C in the preparation. Also disclosed are a method of converting cephalosporin C to glutaryl-7-aminocephalosporanic acid in the absence of exogenous H.sub.2 O.sub.2, and a method of screening for a D-amino acid oxidase-producing microorganism.

Bioconversion of cephalosporin C to glutaryl-7-aminocephalosporanic acid

Growing community of inventors

Kaohsiung, Taiwan

Yun-Huey Lee