Seoul National University

Genechem Inc.

Yun Hee Choi

Sang Mi Lee

Dae Hee Kim

Byung Gee Kim

Byung-Gee Kim

Ji Young Yang

Sun Youp Kang

Won Min Seo

Jin Suk Woo

Jae-Kyung Song

The present invention provides an &#x3b1;-1,3 fucosyltransferase mutant having an increased expression level of soluble protein and increased activity, a DNA encoding the &#x3b1;-1,3 fucosyltransferase mutant, a recombinant vector comprising the DNA encoding the &#x3b1;-1,3 fucosyltransferase mutant, a host cell transformed with the recombinant DNA vector, an extract of the host cell, a method for producing 3-fucosyloligosaccharide, a method for preparing an &#x3b1;-1,3 fucosyltransferase mutant, and a method for screening an &#x3b1;-1,3 fucosyltransferase mutant. The &#x3b1;-1,3 fucosyltransferase mutant of the present invention has a significantly increased soluble protein expression level and activity.

Helicobacter pylori &#x3b1;-1,3 fucosyltransferase gene and protein with improved soluble protein expression and activity, and thereof application for synthesis of &#x3b1;-1,3 fucosyloligosaccharide

The present invention relates to a method for preparing a sialic acid derivative characterized by performing both of a process for preparing CMP-N-acetylneuraminic acid using N-acetyl-D-glucosamine and a process for preparing the sialic acid (neuraminic acid) derivative that combines a sialic acid with a galactose derivative or a lactose derivative, together, in one reactor. According to the method for preparing a sialic acid derivative of the present invention, expensive cytidine 5'-monophosphate (CMP) is capable of being recycled in a reactor, such that an amount of the CMP introduced into the reactor may be reduced, and the sialic acid derivative is capable of being prepared at a significantly high efficiency by using cheap N-acetyl-D-glucosamine, and pyruvate as substrates.

Method for preparing sialic acid derivative

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Seoul, South Korea

Yun Hee Choi