Nantong University

Yun Gu

Xiaosong Gu

Fei Ding

Yumin Yang

Songlin Zhou

Xin Tang

Bin Yu

Leilei Gong

Luzhong Zhang

Hualin Sun

Yongjun Wang

Shiying Li

Yaxian Wang

Hongkui Wang

Chengbin Xue

Tianmei Qian

Provided is a use of one or more MicroRNA genes selected from miRNAs of Family Let-7, miR-21 or miR-222 in the construction of tissue engineered nerves and in the repair of peripheral nerve defects. An outer and/or internal surface or pores of a tissue engineered nerve graft are coated or adsorbed with polymeric nanomicrospheres carrying a Let-7 family miRNA inhibitor, miR-21, or miR-222, or a mimetic thereof, wherein the polymeric material is composed of biocompatible fibronectin and heparin. The regeneration of peripheral nerves and the construction of tissue engineered nerves are promoted by regulating the expression of MicroRNA genes which can effectively promote the proliferation of primary Schwann cells cultured in vitro and have an anti-apoptotic effect on neuronal cells. In-vivo test proves that bridging of the tissue engineered nerve graft can facilitate the regeneration of peripheral nerves, thus being useful in the treatment of peripheral nerve injury.

Construction of MicroRNA gene-mediated novel tissue engineered nerve and applications thereof in repairing nerve defect

A tissue engineered nerve graft for repairing peripheral nerve injury consists of a nerve conduit and a extracellular matrix (ECM) that is secreted by autologous or allogeneic support cells and obtained by decellularization. A preparation method of the ECM-modified tissue engineered nerve grafts containing support cells, nerve conduit and constructing a ECM-modified tissue engineered nerve graft.

Preparation of extracellular matrix-modified tissue engineered nerve grafts for peripheral nerve injury repair

Growing community of inventors

Jiangsu, China

Yun Gu