Jiangsu Academy of Agricultural Sciences

Snu Rdb Foundation

Guotai (Taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou

Yun Bai

Zhixin Feng

Qiyan Xiong

Yuan Gan

Lei Zhang

Zhanwei Wang

Li Wang

Yuzi Wu

Daesub Song

Yanfei Yu

Beibei Liu

Guoqing Shao

Rong Chen

Maojun Liu

Yanna Wei

Jongwoo Lim

Lizhong Hua

Long Zhao

Haiyan Wang

Fei Hao

Fangfang Bai

Minjoo Yeom

Lulu Xu

Guoqinq Shao

Wenliang Li

Yongjie Liu

Daohua Zhang

Xing Xie

Dongxia Liu

A viral vector delivery system for both respiratory and digestive tracts of pigs and an application thereof are provided. The viral vector delivery system includes a backbone plasmid and a helper plasmid. The backbone plasmid is produced by inserting a full-length cDNA of porcine enterovirus B (PEVB) into a pUC57 plasmid. The helper plasmid is produced by inserting a green fluorescent protein-coding gene into a plasmid pCAG-T7-polymerase. The viral vector delivery system can be constructed with high efficiency, can quickly cause a cytopathic effect (CPE) after infecting cells, has a viral titer up to 10TCID/mL, and can maintain high stability. A pathogenic epitope for the respiratory and digestive tracts of pigs is inserted into the backbone plasmid to produce a vaccine antigen, which is non-pathogenic, has high stability and a high viral titer, and allows a prominent immunization effect after being inoculated in pigs.

Viral vector delivery system for both respiratory and digestive tracts of pigs and application thereof

Disclosed are an attenuated live vaccine against mycoplasmal pneumonia of swine (MPS) and use thereof. In the present invention, pathological lung tissues of swine having typical(Mhp) infection and no obvious other pathogenic infections are screened, and subcultured 100 generations in lungs of newborn rabbits; then, Mhp strains are isolated and serially subcultured in a medium; and the Mhp strain AN306 is obtained by screening a plurality of strains, which is deposited with an accession number: CCTCC NO. M2012431. Also disclosed is a live vaccine formulation against MPS prepared on the basis of the attenuated strain and comprising live attenuated strain, a pharmaceutically acceptable carrier or excipient, and optionally an adjuvant and immunogens of other pathogens.

Attenuated live vaccine against mycoplasmal pneumonia of swine (MPS) and use thereof

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Nanjing, China

Yun Bai