Yokogawa Electric Corporation

Yumiko Sugiyama

Takeo Tanaami

Kenta Mikuriya

Saya Satou

Katsumi Isozaki

Hidetoshi Aoki

Hisao Katakura

Yasunori Suzuki

Souichirou Shimoda

Muneki Araragi

A method for measuring a fluorescent light amount, whereby a specimen is irradiated with excitation light, and fluorescent light generated from the specimen is measured by a light-receiving element via of a receiving optical system, the method includes the steps of irradiating the specimen with excitation light having a light-amount value per specified unit area traceable to the national standard, and designated on the surface of the specimen beforehand, causing the light-receiving element to measure the fluorescent light generated from the specimen, and computing a fluorescent light-amount as measured by the light-receiving element of the receiving optical system on the basis of an excitation light-amount per the specified unit area, optical coefficients of the receiving optical system, and the acceptance coefficient of the light-receiving element, thereby working out a light amount value per the unit area traceable to the national standard.

Method for measuring fluorescent light amount or light absorption amount and device for measuring the same

There is provided a molecularity measurement instrument capable of working out the number of molecules in a sample by comparing a measured value of a light quantity with a theoretical light quantity per a single molecule, and a molecularity measurement method using the same. The molecularity of the sample is quantitatively estimated on the basis of a light quantity having correlation with the molecularity. The molecularity measurement method comprises the step of working out a theoretical light quantity per a single molecule, the step of measuring a light quantity of the sample by use of an image detector, and the step of working out the molecularity of the sample on the basis of a ratio of the light quantity of the sample to the theoretical light quantity as worked out.

Molecularity measurement instrument and molecularity measurement method

A biochip for implementing analysis on the basis of the distribution of a quantity of light of fluorescent light generated at sites disposed thereon, the biochip having, markers formed thereon and defined previously in a positional relationship relative to the sites; and a processor for recognizing positions of the sites on the biochip on the basis of the positions of the markers, wherein the markers are formed of at least one of dyes, pigment, metal colloid bonded to biopolymer, dyes bonded to biopolymer, and pigment bonded to biopolymer.

Biochip, biochip reader and biochip reading method

The present invention is characterized by the following points: In a biochip reader used for reading a measurement sample image by light beam irradiation, a correction method for the distribution of quantity of light which is devised to remove the influence of shading for the whole image and such a biochip reader can be realized by correcting non-uniformity in said quantity of light in light beam irradiation by dividing the quantities of light of pixels in a measured image obtained from the measurement of a measurement sample by a distribution of quantity of light in an image obtained from the measurement of a uniform fluorescent plate that presents a uniform fluorescent light distribution, the positions of pixels in the measured image being correspondent to those in the image obtained through the above uniform fluorescent plate measurement.

Correction method for the distribution of quantity of light and biochip-reader

An optical system which corrects an intensity distribution of incident light to a flat intensity distribution includes: a first lens group which includes at least one lens and has a positive refracting power; a second lens group which includes at least one lens and has a negative refracting power, the second lens group being positioned behind the first lens group in a direction of the incident light; and a third lens group which includes at least one lens and has a positive refracting power, the third lens group being positioned behind the second lens group in the direction of the incident light. In the optical system, the incident light is collimated, and the intensity distribution of the incident light is corrected to the flat intensity distribution by spherical aberrations of the first lens group, the second lens group and the third lens group.

Optical system and optical microscope

A site position can be detected using transmitted light or reflected light which does not include an excitation wavelength according to a configuration of a biochip. When reflected light is used, a light sourceis actuated. Light from the light sourcepasses through a barrier filterand is bent by a dichroic mirrorand passes through a dichroic mirrorand illuminates the biochip. The reflected light in the biochipenters a CCD camera. An output signal of the CCD camerais sent to a computation partand a position of a site on the biochipis detected in the computation part

Biochip reading apparatus and biochip reading method

A biochip reader has a microlens plate having a plurality of microlenses, a light source which applies coherent light to the microlens plate as excitation light, a dichroic mirror which transmits or reflects outgoing light from the microlens plate, and reflects or transmits fluorescence occurring on a biochip, a photographing section, a lens which collects the light reflected or transmitted on the dichroic mirror into the photographing section, a barrier filter provided between the dichroic mirror and the photographing section, and a driving section which drives the microlens plate, wherein while the driving section drives the microlens plate, excitation light diverged or collected by the plurality of microlenses scans a surface of the biochip. Each of the microlenses having arbitrary size are provided on the microlens plate at arbitrary positions, An outgoing angle at each microlens of divergence light or collection light is limited to a narrow angle.

Biochip reader

Detector calibration methods are described which enable the measurement of absolute power. The method includes using a power meter with traceability to the national standard for optical power to calibrate the power of each photodetector device of a detector with photodetector device arrays arranged in one dimension or two dimensions and to calibrate the output signals of the detector making it possible to measure the spatial distribution of a light source's power and also values of optical power with traceability to the national standard directly from the output signals of the detector.

Detector calibration method and power measurement instruments using the method

When a plurality of types of biopolymers on a biochip is to be measured using a fluorescence or colorimetric means, the image of the above biochip is obtained by measuring a plurality of images while moving the above biochip step-wise by an integer multiple of the field-of-view of a one-dimensional or two-dimensional array detector on the sample, then by combining the above-described plurality of images.

Biochip measuring method and measuring equipment

The present invention provides a biochip reader for reading with a detector the fluorescence image information from genes to which fluorescent substances are stuck and which are poured into biochip cells, by emitting coherent light beams such as laser light onto the cells as excitation light. The present invention aims to achieve a biochip reader that is small, cheap, and highly durable.

Growing community of inventors

Musashino, Japan

Yumiko Sugiyama