Clontech Laboratories, Inc.

Takara Bio USA, Inc.

Yulii Aleksandrovich Labas

Sergey Anatolievich Lukyanov

Arcady Fedorovich Fradkov

Mikhail V Matz

Alexey Terskikh

Konstantin Anatolyevich Lukyanov

Yuriy Yanushevich

Nadezda Georgievna Gurskaya

Mikhail Vladimirovich Matz

Sergey Anatolievich Lukyanoy

Nucleic acid compositions encoding chromo/fluoroproteins and mutants thereof, as well as the encoded proteins, are provided. The subject proteins of interest are proteins that are colored and/or fluorescent, where this feature arises from the interaction of two or more residues of the protein. Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins. Also provided are fragments of the nucleic acids and the peptides encoded thereby, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications, e.g., that include the subject nucleic acid compositions, are provided.

Nucleic acids encoding chromophores/fluorophores and methods for using the same

Nucleic acid compositions encoding novel chromo/fluoroproteins and mutants thereof are provided. The subject proteins of interest are proteins that are colored and/or fluorescent, where this feature arises from the interaction of two or more residues of the protein. The subject proteins are further characterized in that they are either obtained from non-bioluminescent Cnidarian, e.g., Anthozoan, species or are obtained from non-Pennatulacean (sea pen) species. Specific proteins of interest include proteins obtained from the following specific Anthozoan species:(NFP-1),sp. (NFP-2),sp. (NFP-3 & NFP-4),(NFP-5),sp. 'red' (NFP-6),(NFP-7),sp 'green' (NFP-8), andsp.&#x201c;magenta&#x201d; (NFP-9). Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins. Also provided are fragments of the nucleic acids and the peptides encoded thereby, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications, e.g., that include the subject nucleic acid compositions, are provided.

Nucleic acid compositions encoding novel chromo/fluoroproteins and mutants thereof are provided. The subject proteins of interest are proteins that are colored and/or fluorescent, where this feature arises from the interaction of two or more residues of the protein. The subject proteins are further characterized in that they are either obtained from non-bioluminescent, e.g.,, species or are obtained from non-(sea pen) species. Specific proteins of interest include proteins obtained from the following specificspecies:(NFP-1),sp. (NFP-2),sp. (NFP-3 & NFP-4),(NFP-5),sp. 'red'(NFP-6),(NFP-7),sp 'green'(NFP-8), andsp.&#x201c;magenta&#x201d;(NFP-9). Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins. Also provided are fragments of the nucleic acids and the peptides encoded thereby, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications, e.g., that include the subject nucleic acid compositions, are provided.

Nucleic acid compositions encoding novel chromo/fluoroproteins and mutants thereof, are provided. The subject proteins of interest are proteins that are colored and/or fluorescent, where this feature arises from the interaction of two or more residues of the protein. The subject proteins are further characterized in that they are either obtained from non-bioluminescent Cnidarian, e.g., Anthozoan, species or are obtained from non-Pennatulacean (sea pen) species. Specific proteins of interest include proteins obtained from the following specificspecies:(NFP-1),sp. (NFP-2),sp. (NFP-3 & NFP-4),(NFP-5),sp. 'red' (NFP-6),(NFP-7),sp 'green' (NFP-8), andsp.&#x201c;magenta&#x201d; (NFP-9). Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins. Also provided are fragments of the nucleic acids and the peptides encoded thereby, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications, e.g., that include the subject nucleic acid compositions, are provided.

Nucleic acids encoding chromo or fluorescent proteins and method for using the same

Nucleic acid compositions encoding novel chromo/fluoroproteins and mutants thereof are provided. The subject proteins of interest are proteins that are colored and/or fluorescent, where this feature arises from the interaction of two or more residues of the protein. The subject proteins are further characterized in that they are obtained from non-bioluminescent, e.g.,, species or are obtained from non-(sea pen) species. Specific protiens of interest include protiens obtained from the following specificspecies:(NFP-1),sp. (NFP-2),sp. (NFP-3 & NFP-4),(NFP-5),sp. 'red' (NFP-6),(NFP-7),sp. 'green' (NFP-8), andsp. &#x201c;magneta&#x201d; (NFP-9). Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins. Also provided are fragments of the nucleic acids and the peptides encoded thereby, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications, e.g., that include the subject nucleic acid compositions, are provided.

Nucleic acid compositions encoding novel chromo/fluoroproteins and mutants thereof, are provided. The subject proteins of interest are proteins that are colored and/or fluorescent, where this feature arises from the interaction of two or more residues of the protein. The subject proteins are further characterized in that they are either obtained from non-bioluminescent, e.g.,, species or are obtained from non-Pennatulacean (sea pen) species. Specific proteins of interest include proteins obtained from the following specificspecies:(NFP-1),sp. (NFP-2),sp. (NFP-3 & NFP-4),(NFP-5),sp. 'red' (NFP-6),(NFP-7),'green' (NFP-8), andsp.&#x201c;magenta&#x201d; (NFP-9). Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins. Also provided are fragments of the nucleic acids and the peptides encoded thereby, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications, e.g., that include the subject nucleic acid compositions, are provided.

Nucleic acid compositions encoding novel chromo/fluoroproteins and mutants thereof, are provided. The subject proteins of interest are proteins that are colored and/or fluorescent, where this feature arises from the interaction of two or more residues of the protein. The subject proteins are further characterized in that they are either obtained from non-bioluminescent, e.g.,, species or are obtained from non-(sea pen) species. Specific proteins of interest include proteins obtained from the following specificspecies:(NFP-1),sp. (NFP-2),sp. (NFP-3 & NFP-4),(NFP-5),sp. 'red' (NFP-6),(NFP-7),sp 'green' (NFP-8), andsp.&#x201c;magenta&#x201d; (NFP-9). Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins. Also provided are fragments of the nucleic acids and the peptides encoded thereby, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications, e.g., that include the subject nucleic acid compositions, are provided.

Nucleic acid compositions encoding novel chromo/fluoroproteins and mutants thereof, are provided. The subject proteins of interest are proteins that are colored and/or fluorescent, where this feature arises from the interaction of two or more residues of the protein. The subject proteins are further characterized in that they are either obtained from non-bioluminescent Cnidarian, e.g., Anthozoan, species or are obtained from non-Pennatulacean (sea pen) species. Specific proteins of interest include proteins obtained from the following specific Anthozoan species:(NFP-1),sp. (NFP-2),sp. (NFP-3 & NFP-4),(NFP-5),sp. 'red'(NFP-6),(NFP-7),sp. 'green' (NFP-8), andsp.&#x201c;magenta&#x201d; (NFP-9). Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins. Also provided are fragments of the nucleic acids and the peptides encoded thereby, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications, e.g., that include the subject nucleic acid compositions, are provided.

Nucleic acid compositions encoding novel chromo/fluoroproteins and mutants thereof, are provided. The subject proteins of interest are proteins that are colored and/or fluorescent, where this feature arises from the interaction of two or more residues of the protein. The subject proteins are further characterized in that they are either obtained from non-bioluminescente.g.,species or are obtained from non-(sea pen) species. Specific proteins of interest include proteins obtained from the following specificspecies:(NFP-1),sp. (NFP-2),sp. (NFP-3 & NFP-4),(NFP-5),sp. 'red' (NFP-6),(NFP-7),sp 'green' (NFP-8), andsp. &#x201c;magenta&#x201d; (NFP-9). Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins. Also provided are fragments of the nucleic acids and the peptides encoded thereby, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications, e.g., that include the subject nucleic acid compositions, are provided.

Nucleic acid compositions encoding novel chromo/fluoroproteins and mutants thereof, as well as the proteins encoded the same, are provided. The proteins of interest are proteins that are colored and/or fluorescent, where this feature arises from the interaction of two or more residues of the protein. The subject proteins are further characterized in that they are either obtained from non-bioluminescent, e.g.,, species or are obtained fromnon-(sea pen) species. Specific proteins of interest include the following specific proteins: hcriGFP; dendGFP; zoanRFP; scubGFP1; scubGFP2; rfloRFP; rfloGFP; mcavRFP; mcavGFP; cgigGFP; afraGFP; rfloGFP2; mcavGFP2; and mannFP. Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins. Also provided are fragments of the nucleic acids and the peptides encoded thereby, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications, e.g., that include the subject nucleic acid compositions, are provided.

Chromophores/fluorophores and methods for using the same

Nucleic acid compositions encoding novel chromo/fluoroproteins and mutants thereof, as well as the encoded proteins, are provided. The subject proteins of interest are proteins that are colored and/or fluorescent, where this feature arises from the interaction of two or more residues of the protein. The subject proteins are further characterized in that they are either obtained from non-bioluminescent, e.g., Anthozoan, species or are obtained from non-Pennatulacean (sea pen) species. Specific proteins of interest include proteins obtained from the following specific Anthozoan species:(NFP-1),sp. (NFP-2),sp. (NFP-3 & NFP-4),(NFP-5),sp. 'red'(NFP-6),(NFP-7),sp 'green' (NFP-8), andsp. &#x201c;magenta&#x201d; (NFP-9). Also interest are proteins that are substantially similar to, or mutants of, the above specific proteins. Also provided are fragments of the nucleic acids and the peptides encoded thereby, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications, e.g., that include the subject nucleic acid compositions, are provided.

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Moscow, Russia

Yulii Aleksandrovich Labas