Riken Corporation

Kabushiki Kaisha Dnaform

Yuko Shibata

Yoshihide Hayashizaki

Yasumasa Mitani

Akio Yamane

The present invention relates to a process for synthesizing or amplifying efficiently a nucleic acid comprising a target nucleic acid sequence. The process involves providing a primer comprising in its 3'-end portion a sequence (Ac&#x2032;) which hybridizes a sequence (A) in the 3&#x2032;-end portion of the target nucleic acid sequence, and in the 5&#x2032;-side of the sequence (Ac&#x2032;) a sequence (B&#x2032;) which hybridizes the complementary sequence (Bc) of a sequence (B) positioned in the 5&#x2032;-side of the sequence (A) on the target nucleic acid sequence, wherein {X&#x2212;(Y&#x2212;Y&#x2032;)}/X is in the range of &#x2212;1.00 to 1.00, in which X denotes the number of bases in the sequence (Ac&#x2032;), Y denotes the number of bases in the region flanked by the sequences (A) and (B) in the target nucleic acid sequence, and Y&#x2032; denotes the number of bases in an intervening sequence between the sequences (Ac&#x2032;) and (B&#x2032;) (Y&#x2032; may be zero).

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Kanagawa, Japan

Yuko Shibata