Amano Enzyme Inc.

Suntory Holdings Limited

Yuko Kuritani

Tetsuya Takahashi

Satoru Ishihara

Genichi Nakamura

Naoki Matsumoto

Takako Inui

Tomoko Fujimura

Keita Okuda

Toru Katase

Ikuma Mizuguchi

The usefulness of &#x3b2;-galactosidases derived fromis further enhanced. A modified &#x3b2;-galactosidase in which one or more amino acids selected from the group consisting of proline 182 (P182), tyrosine 187 (Y187), serine 188 (S188), tryptophan 405 (W405), alanine 406 (A406), glutamine 407 (Q407), tyrosine 449 (Y449), threonine 483 (T483), serine 512 (S512), serine 531 (S531), threonine 533 (T533), serine 534 (S534), asparagine 550 (N550), glutamine 551 (Q551), tryptophan 593 (W593), tyrosine 598 (Y598), proline 602 (P602), proline 604 (P604), tyrosine 609 (Y609), lysine 612 (K612), and tyrosine 615 (Y615), or an amino acid(s) corresponding thereto, has/have been substituted by other amino acid in a &#x3b2;-galactosidase consisting of the amino acid sequence of any of SEQ ID NOs. 1 to 4 or an amino acid sequence having 90% or more identity to the amino acid sequence set forth in any of SEQ ID NOs. 1 to 4.

Modified &#x3b2;-galactosidase

It is an object of the present invention to provide a novel enzyme useful for producing low-purine foods or beverages. There is disclosed a nucleosidase comprising an amino acid sequence of SEQ ID NO: 1 or an amino acid sequence having 85% or more identity with the amino acid sequence, or an amino acid sequence of SEQ ID NO: 2 or an amino acid sequence having 88% or more identity with the amino acid sequence.

Nucleosidase

Growing community of inventors

Kakamigahara, Japan

Yuko Kuritani