Sysmex Corporation

Yuko Kawasaki

Hideki Ishihara

Tomoko Matsushima

Satoshi Nakayama

Aya Katayama

Satoshi Tanaka

Masaki Shibayama

Yasuhiro Torikoshi

A CDK profile including a ratio of specific activities of two cyclin dependent kinases closely correlates with the malignancy grade and the presence/absence of the sensitivity to an irritant such as an anticancer agent of a cancer tissue in clinical medicine. Therefore, the properties of a mammalian cell, such as malignancy grade, proliferation potency, sensitivity to an irritant such as an anticancer agent can be assessed based on its CDK profile or by comparing the result of a specific activity ratio of two CDKs with a predetermined threshold corresponding to the ratio. This sensitivity assessing method shows a high ratio of correct assessing results particularly in positive or sensitivity and is useful in predicting efficacy of chemotherapy using the anticancer agent.

Method of assessing properties of mammalian cells, and method of diagnosing cancer using the same

A method for judging feature of malignant tumor is described. The method comprises obtaining step, first comparing step, second comparing step and judging step. The obtaining step comprises obtaining a first parameter based on activity and expression level of a first cyclin dependent kinase (first CDK) contained in a tumor cell of the malignant tumor, a second parameter based on activity and expression level of a second cyclin dependent kinase (second CDK) contained in the tumor cell, a third parameter based on the first parameter and the second parameter, and a fourth parameter based on expression level of a cyclin contained in the tumor cell. The first comparing step comprises comparing a first threshold value with the third parameter. The second comparing comprises comparing a second threshold value with the fourth parameter. The judging step comprises judging the feature of the malignant tumor based on the comparison results of the first comparing step and the second comparing step.

Method for judging feature of malignant tumor

A method for predicting an effectiveness of chemotherapy is described. The method comprises a comparing step and a predicting step. The comparing step is a step of comparing a threshold value with a parameter of a cyclin dependent kinase (CDK) included in a sample containing a tumor cell obtained from a human patient. The predicting step is a step of predicting the effectiveness based on a comparison result of the comparing step. Above stated parameter is selected from the group consisting of an activity, an expression level, and a ratio of activity to expression level.

Method for predicting effectiveness of chemotherapy

A reagent kit that is used for determining a characteristic of tissue obtained from a patient is described. The reagent kit comprising expression measurement reagents for measuring expression level of cyclin-dependant kinase (CDK) and activity measurement reagents for measuring activity value of CDK. The expression measurement reagents comprise first reagents and second reagents, and the activity measurement reagents comprise third reagents and fourth reagents. In the reagent kit, a first reagent set of the first and third reagents is stored under a first storage condition relating to temperature, and a second reagent set of the second and fourth reagents is stored under a second storage condition relating to temperature.

Reagent kit for determining characteristic of tissue

The present invention provides a method for predicting an effectiveness of chemotherapy using an anticancer agent with high probability. The effectiveness is predicted based on a comparison of a parameter relating to CDK and/or CDK inhibitor in a tumor cell specimen obtained from a patient, with a corresponding threshold value to the parameter. The patient to be subjected this sensitivity prediction test of an anticancer agent is administered with the anticancer agent prior to removal of the tumor cell. The threshold is predetermined based on data of correlation between the selected parameter and anticancer agent therapy results obtained from a number of patients actually performed the therapy. Accordingly, the predicting method can provide information useful for determining whether to perform the chemotherapy to individual patients.

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Kobe, Japan

Yuko Kawasaki