Ajinomoto Co., Ltd.

Yuko Kai

Hiroshi Takagi

Mikhail Markovich Gusyatiner

Yuzuru Eto

Tatsuki Kashiwagi

Takami Maekawa

Kohki Ishikawa

Ei-ichiro Suzuki

Mikhail Kharisovich Ziyatdinov

Yutaka Ishiwatari

Ekaterina Igorevna Redkina

By contacting a test substance with a cell that expresses a Kv3.2 protein, and comparing observed cation influx into the cell with cation influx into the cell observed when the test substance is not contacted with the cell, a salty taste modulating substance such as salt alternative compound, or salt perception enhancing or inhibiting compound is screened.

Method for screening a salty taste modulating substance

O-acetylserine, L-cysteine and sulphurous compounds derived therefrom may be produced using a bacterium belonging to the genuswhich harbors a mutant feedback-resistant serine acetyltransferases in which the amino acid sequence corresponding to positions from 89 to 96 in a wild-type serine acetyltransferase is replaced with any one of the amino acid sequences shown in SEQ ID NOS: 4 to 9, and feedback inhibition by L-cysteine in the bacterium is desensitized.

Mutant serine acetyltransferase

Growing community of inventors

Kawasaki, Japan

Yuko Kai