Kyowa Hakko Kogyo Co., Ltd.

Kyowa Hakko Kogyo Kabushiki

Yuko Arai

Makoto Morimoto

Shinzo Ishii

Shigeo Katsumata

Yutaka Saito

Kunikatsu Shirahata

Tadashi Ashizawa

Tadashi Hirata

Motomichi Kono

Keiichi Takahashi

Shigeru Kobayashi

Kazuyuki Mineura

Susumu Tomohiro

Hideo Katsumata

A process for producing mitomycin A by fermentation, which involves culturing a microorganism belonging to the genus Streptomyces, which is capable of producing mitomycin A and having at least one property of (1) deficiency in an ability to produce mitomycin C and (2) a resistance to tryptophan analog, in a culture medium forming and accummulating mitomycin A in the culture medium; and recovering mitomycin A therefrom. The microorganism preferably involves Streptomyces caespitosus T-17-135 (NRRL 12508) or Streptomyces caespitosus (NRRL 12513).

Process and microorganisms for producing mitomycin A by fermentation

A compound represented by the formula: ##STR1## wherein R is a hydrogen atom or a lower alkyl group, X is a hydroxymethyl group and Y is a cyano group, and when R is a lower alkyl group, X and Y may combine to form --CH.sub.2 --O--, and salts thereof, process for preparing the same, and an anti-tumor composition containing the same, which is improved in stability in comparison with a starting compound DC-52.

DX-52-1 compounds and process for preparing the same

A substance designated by us as AX-2 and represented by the formula: ##STR1## This substance is produced by culturing a microorganism of the genus Streptomyces and capable of producing AX-2 in a medium to accumulate AX-2 in the cultured broth and isolating AX-2 therefrom. An anti-tumor composition comprising an effective amount of AX-2 in association with a physiologically acceptable carrier or excipient, which is active against Sarcoma 180.

Substance AX-2, a process for producing the same and an antitumor

Disclosed is a process for purifying an enzyme contained in a solution such as cell extract liquor or fermentation culture liquor. The crude enzyme solution is brought into contact with either a strongly acidic cation exchange resin of high porous type or a strongly basic anion exchange resin of high porous type to adsorb the enzyme on the resin. An eluting agent is then passed through the resin to elute out the enzyme as a purified enzyme solution.

Process for purifying enzymes

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Susono, Japan

Yuko Arai