Toa Medical Electronics Co., Ltd.

Tao Medical Electronics Co., Ltd.

Yukio Tsujino

Yukio Hamaguchi

Takashi Morikawa

Yoshiro Ikeuchi

Berend Houwen

Hitomi Ohmi

Kenji Ito

Kazuhiro Moriyama

Ikuya Takenaka

Improved method for detecting and/or counting the appearance of hematopoietic progenitor cells which comprises the steps of mixing a blood sample with a reagent capable of detecting immature cells without employing any immunological techniques, obtaining cell information about the thus treated sample using a particle analyzer and constructing a cell distribution profile, delineating a portion of the profile as a zone in which at least one subclass of hematopoietic progenitor cells appear, and counting the cells within the zone. The method is capable of detecting the appearance of hematopoietic progenitor cells in a simple and positive manner.

Method of detecting hematopoietic progenitor cells

A reagent for measuring immature leukocytes which comprises: (1) a polyoxyethylene-based nonionic surfactant having the general formula (I) in a sufficient amount capable of fixing cytoplasms and cell membranes of immature leukocytes:

Reagent for measuring immature leukocytes

Reagents for enabling leukocytes to be classified and counted lyse erythrocytes and act on leukocytes to stabilize them are disclosed. The reagents contain polyoxyethylene-based surfactants, and may contain hyperosmotic or hypoosmotic agents and solubilizing agents. One embodiment of the invention contains surfactants selected from the anionic and nonionic polyoxyethylene-based surfactants having 18-30 repeating oxyethylene units per molecule. A second embodiment of the invention contains nonionic polyoxyethylene surfactants only, having 20-100 repeating oxyethylene units per molecule, does not employ either saponin or quaternary ammonium compounds, and provides for adjustment of the pH of the resulting solutions. The invention allows leukocytes to be classified into as many as five types, each type to be quantified, and enables the detection of abnormal cells, when used in conjunction with particulate analysis flow cytometers employing the RF method and the DC method.

Method for classifying leukocytes and a reagent used therefor

A reagent for and method of measuring leukocytes or hemoglobin or both in blood using a reagent which is water-soluble mixture containing:

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Hyogo, Japan

Yukio Tsujino