Mitsubishi Chemical Industries Limited

Agency of Industrial Science and Technology

Ministry of International Trade & Industry

Noda Institute for Scientific Research

Yukio Imada

Katsuhiko Takahashi

Masayuki Kinoshita

Hajime Machida

Daikichiro Nishikawa

Michitaro Nagasawa

Sumiko Mizuno

Yuki Morimoto

Takashi Mikawa

Nobuji Yoshikawa

Tetsu Osozawa

Muconic acid is obtained from the culture of strains of Arthrobacter sp. mutant strains, strains belonging to Corynebacterium acetoacidophilum, Corynebacterium lilium, genus Brevibacterium or genus Microbacterium.

Process for preparing muconic acid

Androstane steroids are produced by microbiological conversion of a sterol substrate with a microorganism belonging to the genus Mycobacterium wherein the medium used contains at least 0.1% by weight of egg yoke as a dry weight.

Process for preparing androstane steroids

9.alpha.-hydroxy-3-oxopregna-4,17(20)-diene-20-carboxylic acid and its methyl ester, and methods of microbiological production thereof, one comprising cultivating a Rhodococcus species in the presence of the corresponding 3-oxopregna-4,17(20)-diene-20-carboxylic acid or its methyl ester, and the other comprising cultivating Mycobacterium species NRRL B-8054 and a Rhodococcus species in the presence of a sterol substrate.

9-.alpha.-Hydroxy steroids

Steroidal alcohols are produced by cultivating a microorganism belonging to the genus Mycobacterium and capable of producing 20 .alpha.-hydroxymethylpregna-1,4-dien-3-one or 20 .alpha.-hydroxymethylpregn-4-en-3-one as a main product in the 859 R

Process for producing steroidal alcohols

The improvement in a process for the production of 17-hydroxyandrosta-1,4-dien-3-one and androsta-1,4-diene-3,17-dione by the microbiological oxidation of a sterol, its 4-en-3-one derivative or its 1,4-dien-3-one derivative, which comprises adding to the culture medium at least one glyceride-containing substance selected from the group consisting of glycerides, fats, oil seeds and oil fruits in an amount sufficient that the culture medium contains at least 0.3 percent by weight of glycerides.

Process for the microbiological oxidation of steroids

Growing community of inventors

Yokohama, Japan

Yukio Imada