Sysmex Corporation

Yukiko Miura

Hiroyuki Kabata

Rena Tsuruoka

Sanai Tsunokuni

Disclosed are devices, programs and methods for determining a type of target peptide contained in a test sample, which relate to comparing first to third information on the target peptide obtained from the test sample with first to third information on a known peptide stored in the storage unit; and determining whether or not the target peptide is the known peptide, wherein the first information is a dissociation rate constant when the target peptide dissociates from a protein, in a solid phase carrier in which the target peptide is immobilized on a support through the protein binding to a peptide, the second information is a maximum value of a layer thickness change amount on the support, and the third information is information indicating that a dissociation mode of a peptide and the protein is either a single dissociation mode or a multiple dissociation mode.

Device, non-transitory computer-readable storage medium, and method for determining type of target peptide

Disclosed is a method of liberating a peptide, the method comprising a step of bringing a self-aggregate of albumin containing a peptide incorporated therein into contact with a solution that contains a compound represented by Formula (I) or (II) defined in the specification at a concentration of 80 mM to 1000 mM and allowing the peptide to be liberated from the self-aggregate of albumin into the solution.

Method of liberating peptide and method of recovering peptide

Disclosed is a method of determining whether a temperature of a sample has reached a predetermined temperature. The method comprises the steps of: mixing an albumin-containing sample with a peptide reagent comprising a peptide for temperature determination; heating the mixture; detecting an optical change of the mixture; and determining whether the temperature of the mixture has reached the predetermined temperature based on the detection result of the optical change. In the method, a dissociation constant (K) of binding of the peptide for temperature determination to albumin is 500 &#x3bc;M or more, and the peptide for temperature determination comprises a first labeling substance and a second labeling substance.

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Kobe, Japan

Yukiko Miura