Idemitsu Kosan Company, Limited

Sds Biotech K.k.

Yuki Tsukagoshi

Yasuyuki Morishita

Takanori Eguchi

An objective of the present invention is to provide a culturing method, by whichbacterial spores can be efficiently produced. The present invention provides a method for producingbacterial spores, comprising culturingbacteria in a liquid medium containing a sugar or a sugar-source raw material at a concentration between 50.1 g/L and 100 g/L at the start of culture, and then in the course of the culture, feeding a fed-batch medium containing a sugar or a sugar-source raw material and a nitrogen-containing compound, and having the weight ratio (C/N ratio) of carbon atoms to nitrogen atoms ranging from 5.5 to 13.5 to the liquid medium.

Method for culturing bacillus bacterium, and method for producing useful substance

A method of producingspores comprising a step of culturing thebacterium using a liquid medium having a C/N ratio (weight ratio of carbon content to nitrogen content) of greater than 4 and less than 9.5.

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Chiyoda-ku, Japan

Yuki Tsukagoshi