Toray Industries, Inc.

Yuki Takii

Masateru Ito

Yoji Ueda

Mai Yagi

Hitoshi Nobumasa

Kunihisa Nagino

Fumio Nakamura

A method evaluates a quality of a dephosphorylation reagent, the method including the steps of: providing a dephosphorylation reagent containing an alkaline phosphatase and a peptide fragment derived from the alkaline phosphatase; and evaluating the dephosphorylation reagent as having a high quality if a content ratio of the peptide fragment to the alkaline phosphatase is a predetermined reference value or less.

Method of evaluating quality of dephosphorylation reagent and method of detecting target nucleic acid

A composition contains an alkaline phosphatase and first to sixth peptide fragments, wherein content ratios of the first to sixth peptide fragments to the alkaline phosphatase satisfy formulas (1) to (6), respectively: (X/Y)×100≤0.6000 (1); (X/Y)×100≤0.1800 (2); (X/Y)×100≤0.2000 (3); (X/Y)×100≤0.8000 (4); (X/Y)×100≤1.6000 (5); and (X/Y)×100≤0.3500 (6), wherein Xto Xrepresent peak area values of the first to sixth peptide fragments calculated by an automatic integration method from an extracted ion chromatogram obtained by an LC-MS/MS analysis of the composition, respectively, and Y represents a peak area value of the alkaline phosphatase calculated by an automatic integration method from a chromatogram obtained by an LC-UV analysis of the composition.

Alkaline phosphatase composition, method of producing dephosphorylated nucleic acid and method of producing labeled nucleic acid

The method of stirring a solution according to the present invention is a method of stirring a solution comprising contacting a selective binding substance immobilized on the surface of a carrier with a solution containing an analyte substance reactive with the selective binding substance, and mixing the fine particles or air bubbles into the solution containing an analyte substance, and moving the fine particles or air bubbles without allowing contact thereof with the selective binding substance-immobilized surface. The present invention provides a stirring method that accelerates the reaction of a carrier-immobilized selective binding substance with an analyte substance and detects a trace amount of analyte at high signal intensity and high S/N ratio. The present invention enables diagnosis and examination in the clinical setting by using a selective binding substance-immobilized carrier such as DNA chip.

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Kamakura, Japan

Yuki Takii