Tokyo Institute of Technology

Institute of Science Tokyo

Yuki Ohmuro

Hiroshi Ueda

Chihiro Miyake

Tetsuya Kitaguchi

Jiulong Su

Tomoya Tsukahara

Provided as a mutant of β glucuronidase suitable for homogeneous immunoassays is a β glucuronidase mutant, wherein, in the amino acid sequence ofβ glucuronidase, methionine at position 516 is substituted with lysine, and tyrosine at position 517 is unsubstituted or substituted with a non-tyrosine aromatic amino acid.

Enzymatic mutant suitable for homogeneous immunoassay method

The purpose of the present invention is to develop a kit capable of detecting antigen or measuring its amount with high sensitivity without requiring an immobilization step and a washing step. The present invention provides a kit for detecting antigen or measuring its amount. The kit comprises a complex of a polypeptide including an antibody light chain variable region and a polypeptide including an antibody heavy chain variable region and a protein M fragment labeled with a fluorescent dye. The protein M fragment is a fragment having a binding ability to the complex.

Kit for detecting antigen or measuring its amount

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Tokyo, Japan

Yuki Ohmuro