Kabushiki Kaisha Dnaform

Riken Corporation

K. K. Dnaform

Yuki Kawai

Yoshihide Hayashizaki

Kengo Usui

Yuki Tanaka

Yasumasa Mitani

Yasumasa Kimura

Kazuhito Nomura

Saori Goda

The present invention provides a primer set including primers that can be designed easily, with which an amplification distance can be shortened. Provided is a primer set for use in a method for isothermally amplifying a target nucleic acid sequence 4. The primer set includes a first primer 1F and a second primer 1R. The first primer 1F includes, on the 3' side thereof, a sequence (A&#x2032;) that can hybridize to a sequence (A) on the 3&#x2032; side of the target nucleic acid sequence. The second primer 1R includes, on the 3&#x2032; side thereof, a sequence (B&#x2032;) that can hybridize to a sequence (B) on the 3&#x2032; side of either a strand extended from the first primer or a complementary strand of the target nucleic acid sequence 4. The first primer 1F and the second primer 1R include, on the 5&#x2032; sides thereof, sequences (C) that are substantially identical to each other.

Primer set for isothermal amplication of a target nucleic acid sequence

The present invention is to provide a pretreatment method that allows RNA to be detected promptly and simply. RNA degradation activity due to lactoferrin present in the human rhinal mucosa is inhibited, for example, by adding iron ion and carbonate ion to a biological sample that contains the human rhinal mucosa. With the pretreated biological sample, an RNA virus gene can be amplified by a reverse transcriptase. Iron ion and carbonate ion can also inhibit reverse transcriptase inhibition due to lysozyme C contained in the human rhinal mucosa. Further, it is preferable to remove the envelope of the RNA virus by adding SDS to the biological sample that contains the human rhinal mucosa.

Pretreatment method of biological sample, detection method of RNA, and pretreatment kit

Problem to be solved There is provided a method for detecting and/or amplifying a nucleic acid contained in a biological sample such as blood or cells conveniently, rapidly, and effectively. Solution There is provided a method for detecting a nucleic acid contained in a sample, comprising the step of adding at least one substance selected from the group consisting of polyphenols, polyhydric alcohols, sugar acids, sugar alcohols, and hydrophilic biodegradable polymers to a sample, the step of complementarily binding an oligonucleotide complementary to a part of the nucleic acid sequence of a nucleic acid to be detected to a part of the nucleic acid sequence, and the step of detecting the nucleic acid to be detected.

Method for detecting and amplifying nucleic acid

Growing community of inventors

Yokohama, Japan

Yuki Kawai