Ajinomoto Co., Ltd.

Yuki Imabayashi

Kunihiko Watanabe

Shunichi Suzuki

Kenzo Yokozeki

Tsuyoshi Nakamatsu

Masakazu Sugiyama

Hiroyuki Nozaki

Hisashi Kawasaki

Sergey Vasilievich Smirnov

Veronika Aleksandrovna Kotliarova

Shinji Kuroda

Natalia Nikolaevna Samsonova

Natalia Yurievna Rushkevich

Tatyana Aleksandrovna Bachina

Olga Sergeevna Beznoschenko

A method for manufacturing a product of a reaction catalyzed by a protein having 2-oxoglutarate-dependent enzyme activity such as (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof using a bacterium transformed with a DNA fragment containing a gene coding for a protein having 2-oxoglutarate-dependent enzyme activity such as L-isoleucine dioxygenase activity; and wherein said bacterium has the ability to produce a product such as (2S,3R,4S)-4-hydroxy-L-isoleucine.

Bacterium producing a product of a reaction catalyzed by a protein having 2-oxoglutarate-dependent enzyme activity and a method for manufacturing the product

The present invention provides genes that encode the N-acetyl-(R,S)-&#x3b2;-amino acid acylases. The N-acetyl-(R,S)-&#x3b2;-amino acid acylases were isolated and purified from bacterial cells and the nucleotide sequences were determined. A host, such as, was used to construct a high-expression system for these genes. The N-acetyl-(R)-&#x3b2;-amino acid acylase produced bysp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 8. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence as shown in SEQ. ID. NO. 7. The N-acetyl-(S)-&#x3b2;-amino acid acylase produced bysp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 10. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence shown inshown in SEQ. ID. NO. 9. The N-acetyl-(R)-&#x3b2;-amino acid acylase produced bysp. AJ110348 (FERM BP-10367) includes, for example, the protein comprised of the amino acid sequence shown in SEQ. ID. NO. 12. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence shown inshown in SEQ. ID. NO. 11.

N-acetyl-(R,S)-beta-amino acid acylase gene

The present invention provides a new method for producing serine derivatives and their optically-activated derivatives in a convenient manner. In the presence of an enzyme, an L-&#x3b1;-amino acid of formula (I): (in the formula (I), Ris a hydrocarbon group)is reacted with an aldehyde of formula (II): (in the formula (II), Ris a hydrocarbon)to produce an L-serine derivative of formula (III). .

Method of producing L-serine derivative and enzyme used therefore

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Kawasaki, Japan

Yuki Imabayashi