Toudai Tlo, Ltd.

Daiichi Pure Chemicals Co., Ltd.

Sekisui Medical Co., Ltd.

Yuka Akimoto

Osamu Miyazaki

Hiroyuki Ebinuma

Takashi Kadowaki

Toshimasa Yamauchi

Hirokazu Yago

Kazuo Hara

Kazunori Saito

Kazuo Kotani

Masanao Matsuo

Yuki Honjo

Osamu Myazaki

Kits and methods for selectively assaying a target adiponectin multimer in a biological sample. Such methods accurately evaluate the relationship between a disease and adiponectin through selective assay of adiponectin multimers and provide information that cannot be obtained through measurement of the total amount of adiponectin alone. A method for selectively assaying a target adiponectin multimer in a biological sample comprising distinguishing target adiponectin multimer from the other adiponectin multimers by using a protease and/or an antibody.

Method of selectively assaying adiponectin multimers

The invention provides a method of pretreating a sample for conveniently, quickly and accurately measuring the total amount of adiponectin present in a biological sample contaminated with various adiponectin multimers. The method of measuring an sample for immunologically assaying the total amount of adiponectin present in the sample comprises reacting, with an adiponectin-containing sample, at least one of a reducing agent, an acid or a salt thereof, a surfactant, and a protease.

Mehod of pretreating sample and immunological assay method using the same

Methods for selectively assaying a target adiponectin multimer in a biological sample. Such methods accurately evaluate the relationship between a disease and adiponectin through selective assay of adiponectin multimers and provide information that cannot be obtained through measurement of the total amount of adiponectin alone. A method for selectively assaying of a target adiponectin multimer in a biological sample comprising distinguishing target adiponectin multimer from the other adiponectin multimers by using a protease and/or an antibody.

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Ryugasaki, Japan

Yuka Akimoto