Mgi Tech Co., Ltd.

Shenzhen Tieweilntelligent Technology Co., Ltd

Yujun Zhou

Wenwei Zhang

Chongjun Xu

Yue Zheng

Yuliang Dong

Zhougang Zhang

Huanhuan Liu

Xing Liu

Xi Zhang

Snezana Drmanac

Scoop

Provided are a group of phi29 DNA polymerase mutants having increased thermal stability and use thereof. The phi29 DNA polymerase mutants are proteins obtained by performing point mutation A and/or point mutation B and/or point mutation C on phi29 DNA polymerase, the point mutation A meaning that an amino acid residue M at position 97 of the phi29 DNA polymerase is mutated to other amino acid residue, the point mutation B meaning that an amino acid residue L at position 123 of the phi29 DNA polymerase is mutated into other amino acid residue, and the point mutation C meaning that an amino acid residue E at position 515 of the phi29 DNA polymerase is mutated to other amino acid residue. The stability of the phi29 DNA polymerase mutants is higher than that of a wild-type phi29 DNA polymerase.

Phi29 DNA polymerase mutant having increased thermal stability and use thereof

Provided are a phi29 DNA polymerase mutant with increased thermo stability, a method for preparing the mutant, the use of the mutant, and a method for increasing the stability of the phi29 DNA polymerase.

PHi29 DNA polymerase mutant with increased thermostability

Growing community of inventors

Shenzhen, China

Yujun Zhou