Meiji Seika Kaisha, Ltd.

Yujiro Yamada

Taro Niida

Takashi Shomura

Norio Ezaki

Tomizo Niwa

Shigeharu Inouye

Shinji Miyadoh

Yasuaki Ogawa

Chuhei Nojiri

Shinji Miyado

Hiroshi Watanabe

Shoichi Amano

Shigeo Seki

Eiichi Akita

Michio Kojima

Yasumitsu Kondo

Jiro Itoh

Kazuo Saito, Deceased

Hitoshi Goi

Mitsugu Itoh

Akira Suzuki

Shinichi Kondo

Takashi Tsuruoka

Katsuyoshi Iwamatsu

Takahiro Ishii

Shigeharu Inoue

Fumio Kai

Shoji Omoto

Takashi Hisamatsu

Takeo Miyazawa

Kazunori Ohba

Yuzo Kazuno

Keinosuke Miyauchi

Saeko Takahashi

Harumi Fukuyashu

Hiromi Toyama

Mitsugu Ito

Kunikazu Totsugawa

Essentially pure plasmids derived from Actinomycetes are described which have at least one restriction cleavage site for specific restriction enzyme and have molecular weight of less than 1.0.times.10.sup.7.

Plasmids derived from Actinomycetes

A 7.alpha.-methoxycephalosporin derivative represented by the formula (I): ##STR1## wherein R.sub.1 represents a heterocyclic ring or an --S-- heterocyclic ring; R.sub.2 represents a hydrogen atom, a carboxy group or a --COOR.sub.5 group wherein R.sub.5 represents a lower alkyl group, a dialkylamino-lower alkyl group or a ##STR2## group wherein R.sub.6 represents a lower alkyl group, a lower acyl group or a lower alkoxycarbonyl group and Y represents a hydrogen atom or a lower alkyl group; R.sub.3 represents a hydrogen atom, a carbamoyl group or a lower acyl group; R.sub.4 represents a hydrogen atom, a lower alkyl group, a dialkylamino-lower alkyl group or a ##STR3## group wherein R.sub.6 and Y are defined as above; A and B, which may be the same or different, each represents a straight chain or branched chain alkylene group having 1 to 5 carbon atoms; and x represents 0 or 1; or a pharmaceutically acceptable salt thereof and a process for producing the same.

7.alpha.Methoxycephalosporin derivatives

A new polymeric compound, MF-300, is provided, which possesses a specific protective activity against bacterial infections caused particularly by Pseudomonas aeruginosa. The compound is derived from the reaction of ribostamycin with crotonoyl chloride followed by concentration of the reaction product and by subsequent recovery therefrom of a fraction having a range of molecular weights of 10,000.about.100,000 when measured by ultrafiltration method.

Polymeric compound, MF-300, with protective activity against bacterial

A novel antibiotic BN-235 substance and a process for making it are described, wherein the hydrochloride thereof has the following properties:

Novel antibiotic BN-235 substance, and process for the production thereof

A novel antibiotic designated BN-183B substance is disclosed which is produced by cultivating a strain of the genus Pseudomonas. The antibiotic has a strong action against Gram-positive and Gram-negative bacteria.

Novel antibiotic, BN-183B substance

A BN-213 substance-producing strain of the genus Pseudomonas is cultivated to produce BN-213 substance in a culture medium. The BN-213 substance is isolated from the culture medium and purified, and proved to be a novel antibiotic.

Novel antibiotic BN-213 substance and its production

L-2-Amino-4-methylphosphinobutyric acid (L-MPGA) which is useful as bactericide and herbicide can be prepared by optical resolution of its racemic mixture, D,L-MPGA with the action of a microbial acylase. The process comprises providing D,L-MPGA in the form of its N-acyl derivative, reacting N-acyl-D,L-MPGA in an aqueous medium with a microbial acylase having an optically specific hydrolytic activity, whereby selectively eliminating the acyl group of N-acyl-L-MPGA to give a mixture of L-MPGA and N-acyl-D-MPGA and isolating L-MPGA from the mixture. The acylase is produced by cultivating a microbial strain belonging to the genus Pseudomonas, Streptomyces or Aspergillus, particularly Pseudomonas maltophilia BN-233, Streptomyces violascens SF 2072, Streptomyces diastatochromogenes SF 2073, Aspergillus sp. KS-101 or Aspergillus sp. KS-102.

Process for the optical resolution of

New antibiotics, SF-2050 substance and SF-2050B substance are produced by cultivating a microorganism Streptomyces sp. SF-2050 now deposited under FERM-P 4358 and under ATCC. 31450 in a liquid culture medium under aerobic conditions, and these antibiotics may be isolated from the fermentation broth and useful as an antibacterial agent. These antibiotics have an activity inhibitory to .beta.-lactamase.

Antibiotics SF-2050 and SF-2050B substances and production and use

An antibiotic BN-200 substance produced by cultivating a BN-200 substance strain of the genus Erwinia and a process for producing the BN-200 substance.

Antibiotic BN-200 substance and process for production thereof

Compounds are provided having the general formula I: ##STR1## wherein X represents an hydroxyl group, an acetoxy group or a thiosulfuric acid residue. The formula I compounds are prepared from compounds of the general formula II: ##STR2## wherein R.sub.1 represents hydrogen, a lower alkanoyl group, an arylalkanoyl group, an alkoxycarbonyl group, a lower haloalkoxycarbonyl group, a substituted or unsubstituted aroyl group, a N-arylcarbamoyl group of a substituted or unsubstituted aryl group; R.sub.2 either represents hydrogen or, together with the ##STR3## group, represents a phthalimido group; and X represents an hydroxyl group, an acetoxy group or a thiosulfuric acid residue, or their metal salts or their salts with organic bases, by causing the culture of a cephalosporin C acylase-producing strain of a microorganism, especially a mold, fungus, or the secondary preparation therefrom, to act on the latter compounds II in the presence of an aqueous medium.

Process for the preparation of 7-amino-cephem compounds using mold fungi

A new antibiotic designated as SF-1623 substance exhibiting a useful antibacterial activity against gram-negative bacteria is now produced by cultivating a new strain ATCC 21999 of the known species Streptomyces chartreusis. The SF-1623 substance in the form of its an alkali metal salt may be recovered from the culture when the cultivation is effected under aerobic conditions in the presence of an alkali metal thiosulfate.

Antibiotic SF-1623 substance and the production thereof

A new antibiotic designated BN-130 substance is now provided, which has antibacterial activity against gram-negative and gram-positive bacteria as well as acid-fast bacteria. BN-130 substance is produced by cultivating a strain of Pseudomanas stutzeri under aerobic conditions.

Antibiotic BN-130 substances and the production thereof

Antibiotic SF-1623 substance

A BN-109 substance producing strain of the genus Bacillus is cultivated under an aerobic condition to produce and accumulate BN-109 substance in a culture medium. The BN-109 substance is isolated from the culture broth and purified, and proved to be a novel antibiotic. The BN-109 is effective in the treatment and prevention of swine scours.

Novel antibiotic BN-109 substance, its production and use

Growing community of inventors

Yokohama, Japan

Yujiro Yamada