Suntory Limited

Daikin Industries, Ltd.

Sigma-Tau Industrie Farmaceutiche Riunite S.p.a.

Biomolecular Engineering Research Institute

Suntory Limited of 1-40, Dojimahama

Yuji Shibano

Teruo Amachi

Toru Nakayama

Yoshikazu Tanaka

Masahiro Nakao

Toshihiko Ashikari

Yukiko Kodama

Norihide Amano

Haruyo Hatanaka

Hajime Yoshizumi

Yasuyoshi Sakai

Norihisa Nakamura

Sakayu Shimizu

Nobuo Kato

Yoshiki Tani

Motoo Sumida

Yoshihide Suwa

Ryutaro Utsumi

Kazumitsu Ueda

Hideko Yomo

Hideyoshi Toyoda

Kazuaki Obata

Hirofumi Koda

Jun Fukushima

Tohru Komano

Hiroto Kondo

Takehiro Matsumoto

Kazuyuki Morihara

Kenji Okuda

Norihisa Kikuchi

Kohei Oda

Process for manufacturing beer

A protein derived from a strain of methylotrophic yeast which has a protein disulfide isomerase activity having the amino acid sequence as set forth in SEQ ID No. 1, or protein in which the amino acid sequence has been modified by deletion or addition of one or a few amino acids, or substitution with other amino acid(s) and which has a protein disulfide isomerase activity; and a process for production thereof.

Protein disulfide isomerase gene derived from strain of methylotrophic

The present invention relates to a novel proteinase inhibitor useful as a medicine or pesticides and a gene coding for the inhibitor. A proteinase inhibitor (Kexstatin) having inhibitory activity against Kex2 proteinase family enzymes and having a molecular weight of 11,500; a gene coding for Kexstatin; and a method for producing Kexstatin using a microorganism belonging to the genus Streptomyces or transformed with Kexstatin gene are disclosed.

Proteinase inhibitor and gene encoding the inhibitor

Described herein are a novel heat-resistant .beta.-galactosyltransferase, a production process of the enzyme and a utilization method of the enzyme. The enzyme is produced preferably by produced by a microorganism belonging to the family of Actinomycetaceae, which may be selected from fungi belonging to the genus Saccharopolyspora, the genus Thermomonospora or the genus Thermoactinomvces.

Heat-resistant .beta.-galactosyltransferase, its production process and

The present invention relates to an expression vector which is induced by methanol and/or glycerol utilizing an alcohol oxidase gene of a methylotrophic yeast; a recombinant methylotrophic yeast containing said vector and capable of accumulating a significantly high amount of an expression product of a heterologous gene; and a method for producing useful products utilizing said recombinant. The expression vector is constructed by utilizing the promoter and terminator of an alcohol oxidase gene of methylotrophic yeast. Further, a significantly high amount of adenylate kinase, cytochrome C552 or peroxidase is produced by using said expression vector.

Vector having promoter that is inducible by methanol and/or glycerol

A collagenase gene derived from bacteria of the species Vibrio alginolyticus is disclosed. A recombinant vector containing the gene, a host cell transformed with a plasmid containing the gene and a process for the production of a collagenase by using the host cells are also disclosed.

Bacterial collagenase gene of Vibrio alginolyticus

A yeast host which can express P-glycoprotein, i.e., the product of MDR-related gene, in the cell membrane in the same state as observed in multidrug resistant cells produced by connecting the MDR-related gene which carries multidrug resistance to a yeast expression vector and transforming the yeast with said recombinant vector; a cell membrane fraction containing a substantial amount of P-glycoprotein produced by said yeast and a process for the preparation thereof; and a recombinant vector for expressing the MDR-related gene in a yeast host.

Expression of MDR-related gene in yeast cell

Fusaric acid resistant genes derived from fusaric acid decomposing or detoxifying microorganisms such as Pseudomonas cepacia and Klebsiella oxytoca are described. Also described are DNA fragments and plasmids, which comprise such fusaric acid resistant genes. Host cells comprising such plasmids are also described.

Fusaric acid resistant genes

A peroxidase gene derived from Arthromyse ramosus and its cloning and expression in E. coli and yeast is disclosed. The peroxidase encoded by this gene is not-specific and does not contain isozymes and hence is suitable as a diagnostic reagent or as a marker enzyme in enzyme innunoassays.

Peroxidase gene of microbial origin

Described herein are a novel heat-resistant .beta.-galactosyltransferase, a production process of the enzyme and a utilization method of the enzyme. The enzyme is produced preferably by produced by a microorganism belonging to the family of Actinomycetaceae, which may be selected from fungi belonging to the genus Saccharopolyspora, the genus Thermomonospora or the genus Thermoactinomyces.

Process for producing novel heat-resistant .beta.-galactosyltransferase

This invention provides a process for producing ethanol from a starchy material, wherein the starchy material is simultaneously subjected to saccharification and subsequent fermentation by use of a yeast host which has been transformed by an expressible recombinant vector comprising a glucoamylase gene derived from a fungus of the genus Rhizopus.

Process for producing alcohol using yeast transformed by Rhizopus

The present invention relates to the Rhizopus derived glucoamylase gene, a novel recombinant vector comprising said gene, and a microorganism transformed by said vector, as well as a process for reproducing Rhizopus glucoamylase by cultivating the transformed microorganism, especially yeast, in a liquid medium.

Glucoamylase gene of rhizopus oryzae

Growing community of inventors

Toyonaka, Japan

Yuji Shibano