Sekisui Chemical Co., Ltd.

Yuji Setoguchi

Kazuyuki Oishi

Toshiki Kawabe

Kazuhiko Shimada

Takayuki Oka

A method for separating hemoglobin A from a hemoglobin mixture according to a cation exchange liquid chromatography characterized that at least two types of eluents are used including an eluent (hereinafter referred to as &ldquo;eluent A&rdquo;) for elution of faster hemoglobins than hemoglobin A and an eluent (hereinafter referred to as &ldquo;eluent B&rdquo;) for elution of hemoglobin A and slower hemoglobins than hemoglobin A , and that the eluent A has a pH of 4.0-6.0 and the eluent B is maintained at a pH of at least 0.5 higher than that of the eluent A.

Method of separating hemoglobin A2

A method for determining hemoglobins is provided which is suitable for determination of stable hemoglobin A . A method for determining hemoglobins by cation exchange liquid chromatography wherein an eluent is used containing a chaotropic ion and further an inorganic acid, an organic acid and/or any salt thereof having a buffer capacity in the 4.0-6.8 pH range.

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Shinnanyo, Japan

Yuji Setoguchi