Amano Pharmaceutical Co., Ltd.

Yuji Nakanishi

Yoshinao Koide

Shotaro Yamaguchi

Teruhiko Beppu

Hitoshi Amano

Satoru Suzuki

Kenichi Takeuchi

Yoshiaki Kurono

This invention provides an L-&agr;-glycerophosphate oxidase (GPO) having excellent properties such as stability, heat resistance and reactivity. A recombinant GPO obtained by replacing an amino acid of a specified position of an amino acid sequence deduced from the,No. 7044 GPO gene or DNA coding for the GPO with other amino acid has excellent thermal stability and reactivity. That is, the invention provides modified forms of the GPO having the amino acid sequence of Sequence No. 1 in the Sequence Listing, in which the 130-position leucine counting from the N-terminus of the GPO is replaced by other amino acid and/or the 225-position serine counting from the N-terminus of the GPO is replaced by other amino acid and/or the 298-position threonine counting from the N-terminus of the GPO is replaced by other amino acid and/or the 420-position aspartic acid counting from the N-terminus of the GPO is replaced by other amino acid.

L-&agr;-glycerophosphate oxidase gene, recombinant DNA, and method for producing modified L-&agr;-glycerophosphate oxidase gene

The present invention provides a novel ascorbate oxidase (ASOD) which catalyzes oxidation reaction of L-ascorbic acid with molecular oxygen to form L-dehydroascorbic acid and hydrogen peroxide, a process for producing the ascorbate oxidase comprising using a microorganism belonging to the genus Eupenicillium, a gene encoding ASOD, a transformant containing such a gene, a process for producing ASOD using such a transformant, and a reagent composition comprising ASOD, such as a reagent composition for examination, a food additive, and a reagent composition in the fields of food and clinical examination. The ascorbate oxidase has excellent stability particularly in a liquid state.

Ascorbate oxidase, gene encoding the same, process for producing the

A recombinant DNA capable of being replicated in a bacterium of the genus Pseudomonas is disclosed. The DNA contains a wide host range plasmid vector having a gene that codes for lipase. A process for producing lipase by transforming a host bacterium with the recombinant DNA is also described.

Recombinant DNA, bacterium of the genus Pseudomonas containing it, and

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Aichi, Japan

Yuji Nakanishi