Leland Stanford Junior University

Yuhong Cao

Nicholas A Melosh

Sergio Leal-Ortiz

Karl Martin Hjort

Chris Rehse

Andy Kah Ping Tay

Amanda Jonsson

Stefanie Rothkoetter

Ryan T Swoboda

Sergio Leal-ortiz

Methods and apparatuses to non-destructively and periodically sample a small quantity of intracellular proteins and mRNA from the same single cell or cells for an extended period of time. Specifically, describe herein are non-perturbative methods for time-resolved, longitudinal extraction and quantitative measurement of intracellular proteins and nucleic acids from a variety of cell types using systems including nanostraws.

Methods of non-destructive nanostraw intracellular sampling for longitudinal cell monitoring

Nanostraws and to methods of utilizing them in order to deliver biologically relevant molecules such as DNA, RNA, proteins etc., into non-adherent cells such as immune cells, embryos, plant cells, bacteria, yeast etc. The methods described herein are repeatedly capable of delivering biologically relevant cargo into non-adherent cells, with high cell viability, dosage control, unaffected proliferation or cellular development, and with high efficiency. Among other uses, these new delivery methods will allow to scale pre-clinical cell reprogramming techniques to clinical applications.

Apparatuses and methods using nanostraws to deliver biologically relevant cargo into non-adherent cells

Described herein are nanostraw well insert apparatuses (e.g., devices and systems) that include nanotubes extending through and out of a membrane so that a material can pass through the membrane from a fluid reservoir depot and into a cell grown onto the nanotubes when electrical energy (e.g., electroporation energy) is applied. In particular, the device, systems and methods described herein may be adapted for cell growth viability and transfection efficiency (e.g., &#x3e;70%). These apparatuses may be readily integratable into cell culturing processes for improved transfection efficiency, intracellular transport, and cell viability.

Nanostraw well insert devices for improved cell transfection and viability

Growing community of inventors

Palo Alto, CA, United States of America

Yuhong Cao